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Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase

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Somatic Cell Genetics

Abstract

The human lymphoblast line MGL8 was treated with HAT and subsequently “mutagenized” with EMS (200 μg/ml) to give 15% survival, and 6-thioguanine-resistant cells were selected by cloning in soft agarose containing the drug (1 μg/ml). Eighteen sublines of independently derived resistant clones were isolated and studied in detail. One subline had a low residual HGPRT activity of about 1% of the parental cells. The HGPRT of this subline had a higher Km for PRPP, was more sensitive to heat, and was degraded faster by trypsin than the enzyme in extracts of MGL8 cells. This resistant subline and three others contained CRM levels of 1-38%, compared to the wild-type, so they probably represent true structural mutants of the HGPRT gene. All the variants maintained the karyotype of the parental line (46, XY, 6p−).

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Authors and Affiliations

  1. Department of Pediatrics, Johns Hopkins University School of Medicine, 21205, Baltimore, Maryland

    J. Epstein & J. W. Littlefield

  2. Department of Internal Medicine, University of Michigan Medical Center, 48104, Ann Arbor, Michigan

    A. Leyva & W. N. Kelley

Authors
  1. J. Epstein
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  2. A. Leyva
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  3. W. N. Kelley
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  4. J. W. Littlefield
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Epstein, J., Leyva, A., Kelley, W.N. et al. Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase. Somat Cell Mol Genet 3, 135–148 (1977). https://doi.org/10.1007/BF01551810

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  • DOI: https://doi.org/10.1007/BF01551810

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