Abstract
The α-l-fucosidases (EC 3.2.1.51) from human and mouse cells could be separated by isoelectric focusing of neuraminidase-treated cell extracts in acrylamide slab gels. Fourteen hybrid clones derived from the fusion of mouse and human cultured fibroblasts and 37 hybrid clones derived from the fusion of human long-term lymphoid lines with mouse RAG cells were tested for expression of human α-l-fucosidase. A strong correlation between the expression of the human enzyme and the presence or absence of human chromosome 1 was found. The presence of human α-l-fucosidase in clones scored as positive by isoelectric focusing was confirmed by Ouchterlony double immunodiffusion against IgG from rabbits immunized with purified human α-l-fucosidase. It is concluded that the structural gene locus for human α-l-fucosidase is located on chromosome 1.
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Turner, B. M., Beratis, N. G., Turner, V. S., and Hirschhorn, K. (1974).Clin. Chim. Acta 57:29–35.
Alhadeff, J. A., Miller, A. L., Wenger, D. A., and O'Brien, J. S. (1974).Clin. Chim. Acta 57:307–313.
Thorpe, R., and Robinson, D. (1975).FEBS Lett. 54:87–92.
Turner, B. M., Turner, V. S., Beratis, N. G., and Hirschhorn, K. (1975).Am. J. Hum. Genet. 27:651–661.
Turner, B. M., Beratis, N. G., Turner, V. S., and Hirschhorn, K. (1975).Nature (London) 257:391–392.
Harris, H., and Hopkinson, D. A. (1976).Handbook of Enzyme Electrophoresis in Human Genetics. North Holland Publishing Co., Amsterdam.
Smith, M., Gold, P., Freedman, S. O., and Shuster, J. (1975).Ann. Hum. Genet. 39:21–31.
Satlin, A., Kucherlapati, R., and Ruddle, F. H. (1975).Cytogenet. Cell Genet. 15:146–152.
Harris, H., and Watkins, J. F. (1965).Nature (London) 205:640–646.
Littlefield, J. W. (1964).Science 145:709–710.
Ham, R. G., and Puck, T. T. (1962).Proc. Soc. Exp. Biol. Med. 111:67–77.
Cuatrecasas, P., and Parikh, I. (1972)Biochemistry 11:2291–2299.
Weir, D. M. (1967).Handbook of Experimental Immunology. F. A. Davis Co., Philadelphia.
Turner, V. S., Turner B. M., Kucherlapati, R., Ruddle, F. H., and Hirschhorn, K. (1976).Baltimore Conference (1975): Third International Workshop on Human Gene Mapping. Birth Defects: Original Article Series, XII, 7. The National Foundation-March of Dimes, New York, pp. 238–240.
Corney, G., Fisher, R. A., Cook, P. J. L., Noades, J., and Robson, E. B. (1977).Ann. Hum. Genet. 40:403–405.
Hamerton, J. L. (1976).Baltimore Conference (1975): Third International Workshop on Human Gene Mapping. Birth Defects: Original Article Series XII, 7. The National Foundation-March of Dimes, New York, pp. 7–23.
Ya Wiederschain, G., Kolibaba, L. G., and Rosenfeld, E. L. (1973).Clin. Chim. Acta 46:305–310.
Robinson, D., and Thorpe, R. (1973).Clin. Chim. Acta 47:403–407.
Alhadeff, J. A., Miller, A. L., Wenaas, H., Vedvick, T., and O'Brien, J. S. (1975).J. Biol. Chem. 250:7106–7113.
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Turner, B.M., Smith, M., Turner, V.S. et al. Assignment of the gene locus for human α-l-fucosidase to chromosome 1 by analysis of somatic cell hybrids. Somat Cell Mol Genet 4, 45–54 (1978). https://doi.org/10.1007/BF01546492
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DOI: https://doi.org/10.1007/BF01546492