Abstract
The influence of carbon, nitrogen, and phosphate source, along with concentration, was determined for effect on the weight average molecular weight, molecular weight distribution, and yield of pullulan produced byAureobasidium pullulans NRRL-Y 6220. Batch systems, scale-up batch, and continuous fermentations of 1 L and 10 L were also evaluated as were processing variables, including solvents, and extraction conditions. Products with weight average molecular weight from 1.0 × 105 to 4.0 × 106 were produced in 100-g quantities by varying fermentation conditions such as constituents of the culture medium, pH, and length of incubation. Three sets of culture conditions were defined for the formation of low (<5.0×105), medium (1.0–2.0×106), and high (>2.0×106) weight average molecular weight polymer. These defined molecular weight fractions of pullulan were used in further studies in producing films and fibers.
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Wiley, B.J., Ball, D.H., Arcidiacono, S.M. et al. Control of molecular weight distribution of the biopolymer pullulan produced byAureobasidium pullulans . J Environ Polym Degr 1, 3–9 (1993). https://doi.org/10.1007/BF01457648
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DOI: https://doi.org/10.1007/BF01457648