Summary
Two conditions of in vitro translation are described in which the RNA of Rous sarcoma virus is significantly less efficient at protein synthesis than host cellular chick mRNA. In direct competition, addition of increasing amounts of host cell mRNA inhibits synthesis ofgag gene products from RSV RNA. When the denaturant dimethyl sulfoxide is added to a mixture of the two RNA's the inhibition is even greater. These results support the idea that protein synthesis from avian retrovirus RNA is negatively regulated in vivo in the presence of the host cell mRNA.
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Dedicated to Dr. Wolfgang K. Joklik on the occasion of a symposium entitled “Opening New Vistas in Virology”, held June 16, 1988 in Austin, Texas, celebrating his contributions to the field of virology.
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Borchelt, D.R., Perdue, M.L. Inhibition of avian retrovirus protein synthesis in the presence of host cellular mRNA. Archives of Virology 107, 261–271 (1989). https://doi.org/10.1007/BF01317922
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DOI: https://doi.org/10.1007/BF01317922