Summary
Two serological tests were used to examine the antigenic relationships between murine hepatitis viruses that cause different diseases in mice. Antisera prepared by immunization of mice with the individual viruses were tested for their ability to neutralize both the homologous immunogen and the other viruses. By a plaque reduction neutralization test, each antiserum was found to be specific for the immunizing virus; however, there was substantial cross-reactivity, indicating the viruses were closely related. By kinetic neutralization, two of the viruses tested, MHV-JHM and MHV-2, were found to be antigenically distinct. MHV-3 and MHV-A59 were found to be antigenically very similar but distinct. These data show that kinetic neutralization is a more precise method for determining the antigenic relationships between murine coronaviruses.
Article PDF
Similar content being viewed by others
References
Collins, A. R., Knobler, R. L., Powell, H. R., Buchmeier, M. J.: Monoclonal antibodies to murine hepatitis virus-4 (Strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. Virology119, 358–371 (1982).
Gledhill, A. W., Niven, J. S. F.: Latent virus as exemplified by mouse hepatitis virus (MHV). Vet. Rev. Annot.1, 82–90 (1955).
Hierholzer, J. C., Broderson, J. R., Murphy, F.: New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Infect. Immun.24, 508–522 (1979).
Hirano, N., Fujiwara, K., Hino, S., Matumoto, M.: Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture. Arch. Virol.44, 298–302 (1974).
Karjalainen, K., Makela, O.: A Mendelian idiotype is demonstrable in the heteroclitic anti-NP antibodies of the mouse. Eur. J. Immunol.8, 105–112 (1978).
Lai, M. M. C., Stohlman, S. A.: The RNA of mouse hepatitis virus. J. Virol.26, 236–242 (1978).
Lai, M. M. C., Stohlman, S. A.: Comparative analysis of RNA genomes of mouse hepatitis viruses. J. Virol.38, 661–670 (1981).
Lai, M. M. C., Brayton, P. R., Armen, R. C., Patton, C., Pugh, C., Stohlman, S. A.: Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3. J. Virol.39, 823–834 (1981).
Robb, J., Bond, C. W.: Pathogenic murine coronaviruses. I. Characterization of biological behaviorin vitro and virus-specific intracellular RNA of strongly neurotropic JHM and weakly neurotropic A59 viruses. Virology94, 352–370 (1979).
Siddell, S., Wege, H., ter Meulen, V.: The structure and replication of coronaviruses. Curr. Topics Microbiol. Immunol.99, 131–163 (1982).
Stohlman, S. A., Eylar, O. R., Wisseman, C. L.: Dengue viral antigens in host cell membranes. Acta Virol.22, 31–36 (1978).
Stohlman, S. A., Frelinger, J. A., Weiner, L. P.: Resistance to fatal central nervous system disease by mouse hepatitis virus, strain JHM. II. Adherent cell mediated protection. J. Immunol.124, 1733–1739 (1980).
Stohlman, S. A., Weiner, L. P.: Chronic central nervous system demyelination in mice after JHM virus infection. Neurol.31, 38–44 (1981).
Taguchi, F., Makino, S., Fujiwara, K.: Antigenic differentiation of mouse hepatitis virus by neutralization test. Microbiol. Immunol.26, 741–745 (1982).
Wege, H., Stephanson, R., Koga, M., Wege, H., ter Meulen, V.: Genetic variation of neutropic and non-neurotropic murine coronaviruses. J. gen. Virol.54, 67–74 (1981).
Wege, H., Siddell, S., ter Meulen, V.: The biology and pathogenesis of coronaviruses. Curr. Topics Microbiol. Immunol.99, 165–200 (1982).
Author information
Authors and Affiliations
Additional information
With 1 Figure
Rights and permissions
About this article
Cite this article
Childs, J.C., Stohlman, S.A., Kingsford, L. et al. Antigenic relationships of murine coronaviruses. Archives of Virology 78, 81–88 (1983). https://doi.org/10.1007/BF01310860
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310860