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Ultrastructural localization of carbohydrate in the hymenium and subhymenium ofCoprinus

Evidence for the function of the Golgi apparatus

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Summary

The role and distribution of the Golgi apparatus has been compared in hymenial and subhymenial cells ofCoprinus cinereus using conventional electron microscopic and carbohydrate localization techniques. Basidia at early interphase II of meiosis possessed numerous single Golgi cisternae. Golgi vesicles may contain fibrous granular material similar to basidial and basidiospore walls. Vesicles similar in size and apparently in content to those on cisternae accumulated at the growing apex of the young basidiospore. Golgi vesicles were also found in cystidia but appeared to be absent in other cells studied. Pseudoparaphyses, cystidia and subhymenial cells contained large deposits of glycogen which were removed enzymatically in light microscope preparations, but carbohydrate staining persisted in the cytoplasm of basidia, cystidia and basidiospores at the probably sites of Golgi vesicles and cisternae after enzymatic digestion. Septal pore caps of subhymenial cells were surrounded by a fibrillar cytoplasmic zone devoid of cell organelles except ribosomes. The periodic acid-silver hexamine and silver protein techniques for ultrastructural localization of carbohydrates were compared; the latter gave specific results with the controls used. Carbohydrates were localized in certain wall layers of the immature basidiospore and in the contents of Golgi vesicles. Staining also occurred in glycogen, plasma membrane and lomasomes. In the septal pore apparatus staining occurred only in the septal wall and a region in the septal pore swelling probably containing fibrils. The wall of the pseudoparaphyses stained more than the basidial wall. The results suggest that carbohydrates accumulate in Golgi vesicles attached to cisternae, that changes in vesicle contents occur during migration to the basidiospore, and that these vesicles may transport polysaccharides and mucopolysaccharides to the developing basidiospore wall.

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  1. David J. McLaughlin

    Present address: Department of Botany, 220 Biological Sciences Center, University of Minnesota, St. Paul, Minnesota, USA

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    McLaughlin, D.J. Ultrastructural localization of carbohydrate in the hymenium and subhymenium ofCoprinus . Protoplasma 82, 341–364 (1974). https://doi.org/10.1007/BF01275728

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