Summary
Chronically denervated rat and rabbit tibial nerve distal stumps were studied 3–58 weeks following nerve transaction. Schwann cells, macrophages and possibly fibroblasts participated in myelin removal which was largely complete by seven weeks. Degenerating myelinated and unmyelinated fibres developed respectively into circular and flattened columns of Schwann cell processes each delimited by a basal lamina. Schwann cell columns became encircled by fibroblasts and later by cells of perineurial type, underwent shrinkage with time and eventually were replaced by connective tissue. In another experiment, endoneurial tissue was removed from rabbit tibial nerve stumps seven weeks after transection and transplanted between the corneal stroma of the same animal for 2–6 weeks. In this locus, Schwann cells developed a thickened basal lamina and then underwent necrosis. It was concluded that the maintenance of Schwann cells in bands of Büngner is in part dependent on axonal contact and that failure of reinnervation eventually causes the columns of Schwann cells to disappear.
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Abercrombie, M. andJohnson, M. L. (1946a) Quantitative histology of Wallerian degeneration. I. Nuclear population in rabbit sciatic nerve.Journal of Anatomy 80, 37–50.
Abercrombie, M. andJohnson, M. L. (1946b) Collagen content of rabbit sciatic nerve during Wallerian degeneration.Journal of Neurology and Psychiatry 9, 113–8.
Abercrombie, M. andJohnson, M. L. (1947) The effect of reinnervation on collagen formation in degenerating sciatic nerves of rabbit.Journal of Neurology and Psychiatry 10, 89–92.
Aguayo, A. J., Charron, L. andBray, G. M. (1976a) Potential of Schwann cells from unmyelinated nerves to produce myelin: a quantitative ultrastructural and radioautographic study.Journal of Neurocytology 5, 565–73.
Aguayo, A. J., Epps, J., Charron, L. andBray, G. M. (1976b) Multipotentiality of Schwann cells in cross-anastomosed and grafted myelinated and unmyelinated nerves: quantitive microscopy and radioautography.Brain Research 104, 1–20.
Aguayo, A. J., Peyronnard, J. M., Terry, L. C., Romine, J. S. andBray, A. M. (1976c) Neonatal neuronal loss in rat superior cervical ganglia: retrograde effect on developing preganglionic axons and Schwann cells.Journal of Neurocytology 5, 137–55.
De La Motte, D. J., Hall, S. M. andAllt, G. (1975) A study of the perineurium in peripheral nerve pathology.Acta Neuropathologica 33, 257–70.
Donat, J. R. andWiśniewski, H. M. (1973) The spatio-temporal pattern of Wallerian degeneration in mammalian peripheral nerves.Brain Research 53, 41–53.
Gamble, H. J. andBreathnach, A. S. (1965) An electron-microscope study of human foetal peripheral nerves.Journal of Anatomy 99, 573–84.
Holmes, W. andYoung, J. Z. (1942) Nerve regeneration after immediate and delayed suture.Journal of Anatomy 77, 63–96.
Hogan, M. J., Alvardo, J. A. andWeddell, J. E. (1971)Histology of the Human Eye. Philadelphia: W. B. Saunders.
Holtzman, E. andNovikoff, A. B. (1965) Lysosomes in the rat sciatic nerve following crush.Journal of Cell Biology 27, 651–69.
Kaye, G. I., Donahue, S. andPappas, G. D. (1963) Electron microscopical evidence for the uptake of colloidal particles by Schwann cellsin situ.Journal de Microscopie 2, 605–20.
Masson, P. (1942) Tumeurs encapsulés et benignes des nerfs.Revue Canadienne de Biologie 209–343.
McCarthy, K. D. andPartlow, L. M. (1976) Neuronal stimulation of [3H] thymidine incorporation by primary cultures of highly purified non-neuronal cells.Brain Research 114, 415–26.
Morris, J. H., Hudson, A. R. andWeddell, G. (1972) A study of degeneration and regeneration in the divided rat sciatic nerve based on electron microscopy. IV. Changes in fascicular microtopography, perineurium and endoneurial fibroblasts.Zeitschrift für Zellforschung und mikroskopische Anatomie 124, 165–203.
O'Daly, J. A. andImaeda, T. (1967) Electron microscopic study of Wallerian degeneration in cutaneous nerves caused by mechanical injury.Laboratory Investigation 17, 744–66.
Palmer, E., Rees, R. J. W. andWeddell, G. (1961) The phagocytic activity of Schwann cells. InCytology of Nervous Tissue, p. 49 London: Taylor and Francis.
Spencer, P. S. (1974) The traumatic neuroma and proximal stump.Bulletin of the Hospital for Joint Diseases 35, 85–102.
Spencer, P. S. andWeinberg, H. J. (1978) Axonal specification of Schwann cell expression and myelination. InPhysiology and Pathobiology of Axons (edited byWaxman, S.), pp. 231–239. New York: Raven Press.
Spencer, P. S., Weinberg, H. J., Raine, C. S. andPrineas, J. W. (1975) The perineurial window — a new model of focal demyelination and remyelinationBrain Research 96, 323–9.
Sunderland, S. andBradley, K. C. (1950a) Denervation atrophy of the distal stump of a severed nerve.Journal of Comparative Neurology 93, 401–10.
Sunderland, S. andBradley, K. C. (1950b) Endoneurial tube shrinkage in the distal segment of a severed nerve.Journal of Comparative Neurology 93, 411–20.
Thomas, P. K. (1964) The deposition of collagen in relation to Schwann cell basement membrane during peripheral nerve regeneration.Journal of Cell Biology 23, 375–82.
Thomas, P. K. (1966) The cellular response to nerve injury. I. The cellular outgrowth from the distal stump of transected nerve.Journal of Anatomy 100, 287–303.
Thomas, P. K. (1974) Nerve Injury. InEssays on the Nervous System. A Festschrift for Professor J. Z. Young (edited byBellairs, R. andGray, E. G.), pp. 44–70, Oxford: Clarendon Press.
Thomas, P. K. andJones, D. G. (1967) The cellular response to nerve injury. Regeneration of the perineurium after nerve section.Journal of Anatomy 101, 45–55.
Weinberg, E. L. andSpencer, P. S. (1978) Signalling of central myelination by regenerating peripheral axons.Brain Research (in press).
Weinberg, H. J. andSpencer, P. S. (1975) Studies on the control of myelinogenesis. 1. Myelination of regenerating axons after entry into a foreign unmyelinated nerve.Journal of Neurocytology 4, 395–418.
Weinberg, H. J. andSpencer, P. S. (1976) Studies on the control of myelinogenesis. II. Evidence for neuronal regulation of myelin production.Brain Research 113, 363–78.
Weiss, P. andWang, H. (1945) Transformation of adult Schwann cells into macrophages.Proceedings of the Society for Experimental Biology and Medicine 58, 273.
Williams, P. L. andHall, S. M. (1971) Chronic Wallerian degeneration — Anin vivo and ultrastructural study.Journal of Anatomy 109, 487–503.
Wood, P. M. andBunge, R. P. (1975) Evidence that sensory axons are mitogenic for Schwann cells.Nature 256, 662–4.
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Weinberg, H.J., Spencer, P.S. The fate of Schwann cells isolated from axonal contact. J Neurocytol 7, 555–569 (1978). https://doi.org/10.1007/BF01260889
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DOI: https://doi.org/10.1007/BF01260889