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Mapping and characterization of 129 cosmids on human chromosome 11p

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Somatic Cell and Molecular Genetics

Abstract

We constructed cosmid libraries from human-hamster somatic cell hybrids that possess all or part of the short arm of chromosome 11 as their only human complement and isolated 129 human 11p clones. These cosmids map to 22 of 25 intervals distinguished by a hybrid panel for chromosome 11p. Forty-eight single-copy sequences were subcloned from 25 cosmids. Six of 17 (35%) single-copy sequences tested identify 11 new polymorphisms. Restriction endonuclease analysis identified CpG islands in 16 of 68 cosmids (23.5%). Analysis of the distribution of restriction endonuclease sites recognizing CpG dinucleotides showed that clusters of these sites, including those associated with the 5′ region of an 11p13 Wilms' tumor gene,WT1, can span greater distances than generally recognized. The cosmids reported here should contribute to the construction of long-range physical maps and the isolation of additional genes on the short arm of chromosome 11.

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Authors and Affiliations

  1. Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, Massachusetts

    Katherine M. Call, Charles Lindberg, Asli Memisoglu & Constantia Petrou

  2. Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

    Caryn Y. Ito

  3. Department of Medicine, Harvard Medical School, Boston, Massachusetts

    Tom Glaser

  4. E. Roosevelt Institute for Cancer Research, Denver, Colorado

    Carol Jones

  5. Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts

    David E. Housman

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  1. Katherine M. Call
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Call, K.M., Ito, C.Y., Lindberg, C. et al. Mapping and characterization of 129 cosmids on human chromosome 11p. Somat Cell Mol Genet 18, 463–475 (1992). https://doi.org/10.1007/BF01233086

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  • DOI: https://doi.org/10.1007/BF01233086

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