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Enhanced embryo development of rabbit oocytes fertilized in vitro with platelet activating factor (PAF)-treated spermatozoa

  • Animal Investigations
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Purpose

The purpose of this study was to determine the effects of PAF treatment of rabbit spermatozoa on in vitro fertilization and subsequent blastocyst formation. Rabbit spermatozoa were exposed to PAF (10 −7 M), lyso-PAF (10 −7 M), or HIS (385 mOsm/kg) for 15 min prior to insemination of ovulated oocytes. Fertilized oocytes were cultured to the hatched blastocyst stage.

Results

Fertilization rates with PAF were significantly higher than those of fresh (P <0.001), lyso-PAF-treated (P <0.01), HIS-treated (P <0.05) spermatozoa. Two-cell embryos produced from oocytes inseminated with PAF-treated spermatozoa had significantly higher hatched blastocysts than oocytes inseminated with fresh (P <0.01), lyso-PAF-treated (P <0.05), or HIS-treated (P <0.05) spermatozoa.

Conclusion

We conclude that PAF treatment of spermatozoa increases fertilization rates and subsequent embryonic development.

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Author notes

  1. Brijinder S. Minhas

    Present address: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, The University of Tennessee, Memphis, 956 Court Avenue, Rm. D-328, 38163, Memphis, Tennessee, USA

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee, USA

    William E. Roudebush, Aisaku I. Fukuda & Brijinder S. Minhas

Authors
  1. William E. Roudebush
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  2. Aisaku I. Fukuda
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  3. Brijinder S. Minhas
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Roudebush, W.E., Fukuda, A.I. & Minhas, B.S. Enhanced embryo development of rabbit oocytes fertilized in vitro with platelet activating factor (PAF)-treated spermatozoa. J Assist Reprod Genet 10, 91–94 (1993). https://doi.org/10.1007/BF01204447

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  • DOI: https://doi.org/10.1007/BF01204447

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