Abstract
Cryopreserved embryos should survive centuries. Upon rederivation,in vitro survival is high. Upon reimplantation,in vivo survival is substantial. Genetic stability of the embryos at −196°C is theoretically absolute for several decades and likely high for centuries. Cryopreservation and rederivation services are available at a reasonable cost from commercial vendors. Preservation of a sufficient number of embryos can serve as an insurance policy against loss of the lines from several causes. In addition to the protective utility of a single banked sample, repeated preservation during the course of selection offers several advantages. Correlated responses to selection which appear late in selection may be verified by use of a cross-sectional experimental design to take a “snapshot” of the gradually developing response. After attainment of the selection limit, a generation may be chosen retrospectively which represents the best compromise between maximizing response and minimizing inbreeding irrelevant to the selected trait. Estimates of changes in gene frequencies could be derived from molecular biological analyses. An example of such an experiment in progress employing embryos from the COLD and HOT selected mouse lines is discussed.
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Crabbe, J. C., Kosobud, A., Tam, B. R., Young, E. R., and Deutsch, C. M. (1987). Genetic selection of mouse lines sensitive (COLD) and resistant (HOT) to acute ethanol hypothermia.Alcohol Drug Res. 7:163–174.
Crabbe, J. C., Feller, D. J., and Dorow, J. S. (1989). Sensitivity and tolerance to ethanol-induced hypothermia in genetically selected mice.J. Pharmacol. Exp. Ther. 249:456–461.
Feller, D. J., and Crabbe, J. C. (1991). Effect of alcohols and other hypnotics in mice selected for differential sensitivity to the hypothermic actions of ethanol.J. Pharmacol. Exp. Ther. 256:947–953.
Feller, d. J., Riggan, J., and Crabbe, J. C. (1993). Serotonin and genetic differences in sensitivity and tolerance to ethanol hypothermia.Psychopharmacology (in press).
Kiianmaa, K., Hyytiä, P., and Sinclair, J. D. (1992). Development of an animal model of ethanol abuse: genetic approach. In Boulton, A. A., Baker, G. B., and Wu, P. H. (eds.),Neuromethods Vol. 24, Animal Models of Drug Addiction, Humana Press, Totowa, N.J., pp. 29–63.
Leibo, S. P., and Mazur, P. (1978). Methods for the preservation of mammalian embryos by freezing. In McDaniel, J. C. (ed.),Methods in Mammalian Reproduction, Academic Press, New York, pp. 179–201.
Litt, M., and Luty, J. A. (1989). A hypervariable microsatellite revealed byin vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene.Am. J. Hum. Genet. 44:397–401.
Mazur, P. (1976). Freezing and low temperature storage of living cells. In Mühlbock, O. (ed.),Proceedings of the Workshop on Basic Aspects of Freeze Preservation of Mouse Strains, Gustav Fischer Verlag, Stuttgart, pp. 1–12.
Mazur, P. (1984). Freezing of living cells: Mechanisms and implications.Cell Physiol. 16:C125-C142.
Mazur, P., Cole, K. W., Hall J. W., Schreuders, P. D., and Mahowald, A. (1992). Cryobiological preservation ofDrosophila embryos.Science 258:1932–1935.
Phillips, T. J., and Crabbe, J. C. (1991). Behavioral studies of genetic differences in alcohol action. In Crabbe, J. C., and Harris, R. A. (eds.),The Genetic Basis o Alcohol and Drug Actions, Plenum, New York, pp. 25–104.
Phillips, T. J., Terdal, E. S., and Crabbe, J. C. (1990). Response to selection for sensitivity to ethanol hypothermia: Genetic analyses.Behav. Genet. 20:473–480.
Pomeroy, K. O. (1991). Cryopreservation of transgenic mice.Genet. Anal. Tech. Appl. 8:95–101.
Roubertoux, P. L., Moutier, R., and Toyama, K. (1992). Elimination of maternal effect to demonstrate that embryo cryopreservation is not negligible and interacts with genotype.Behav. Genet. 22:750 (abstr.).
Schneider, U., and Mazur, P. (1986). Implications and applications of the long-term preseration of embryos by freezing. In Morrow, D. (ed.),Current Therapy in Theriogenology II W. B. Saunders, Philadelphia, pp. 81–83.
Sinclair, J. D. (1992). Reply to commentators.Behav. Genet. 22:35–42.
Weber, J. L., and May, P. E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.Am. J. Hum. Genet. 44:388–396.
Whittingham, D. G., Leibo, S. P., and Mazur, P. (1972). Survival of mouse embryos frozen to −196° and −269°C.Science 178:411–414.
Wilmut, I. (1972). The effect of cooling rate, warming rate, cryoprotective agent and stage of development on survival of mouse embryos during freezing and thawing.Life Sci. 11:1071–1079.
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Crabbe, J.C., Schneider, U., Hall, J.W. et al. Invited commentary: Cryopreservation as a tool for the study of selectively bred lines in rodent behavioral genetics. Behav Genet 23, 307–312 (1993). https://doi.org/10.1007/BF01067430
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DOI: https://doi.org/10.1007/BF01067430