Accumulation kinetics of drugs with nonlinear plasma protein and tissue binding characteristics

Abstract

The purpose of this investigation was to study, by digital computer simulation, the accumulation kinetics of drugs which exhibit concentration-dependent binding to tissues and either linear (constant free fraction) or concentration-dependent (increasing free fraction with increasing drug concentration) binding to plasma proteins. It was assumed that elimination rate is proportional to free drug concentration in plasma and that there occurs instantaneous equilibration of drug between vascular and nonvascular spaces. Nonlinear binding can yield, under certain conditions, apparently biexponential plasma concentrationtime curves which may be misinterpreted as being representative of a linear and biexponential-system. Such misinterpretation would cause the following errors in the prediction of drug accumulation and elimination kinetics during and after constantrate infusion: (a) the time required to reach steady state may be overestimated, and (b) the prominence of the apparent distribution phase after cessation of infusion may be underestimated. Drugs with linear and nonlinear plasma protein binding characteristics differ with respect to the relationship between infusion rate and steadystate concentration. This relationship is linear when plasma protein binding is linear. Steadystate concentration increases less than proportionally with increasing infusion rate if plasma protein binding is drug concentration dependent.