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Immobilization ofArthrobacter simplex cells in thermally reversible hydrogels: Comparative effects of organic solvent and polymeric surfactant on steroid conversion

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Summary

Organic solvents have sometimes been used to increase the solubility of water insoluble substrates for steroid transformation using immobilized whole cells, even though the cell viability is often damaged. Polymeric surfactants which form micelles in aqueous solutions could be used instead of organic solvents to solubilize the steroid. We have successfully utilized this approach by employing a poly(dimethyl siloxane)-poly(ethyleneoxide) (PDMS-PEO) block copolymer surfactant to enhance conversion of hydrocortisone to prednisolone by immobilizedArthrobacter simplex cells, without deactivation of the immobilized cells.

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References

  • Ceen, E. G., Herrmann, J. P. R., and Dunhill, P. (1987).Appl. Microbiol. Biotechnol.,25, 491–494.

    Google Scholar 

  • Carrea, G., Colombi, F., Mazzola, G., Cremonesi, P., and Antonini, E. (1979),Biotech. Bioeng.,21, 39–48.

    Google Scholar 

  • Dong, L. C. and Hoffman, A. S. (1986).J. Controlled Rel.,4, 223–227.

    Google Scholar 

  • Dong, L. C. and Hoffman, A.S. (1987). “Thermally Reversible Gels IV: Swelling and Deswelling Characteristics of Copoly(NIPAAm/AAm) Gels Containing Immobilized Enzymes” inACS Symposium Series 350, “Reversible Polymeric Gels and Related Systems”, P. Russo, ed., pp. 236–244, ACS, Wash. D.C..

    Google Scholar 

  • Fukui, S., Tanaka, A., and Iida, T. (1987). “Immobilization of Biocatalysts for Bioprocess in Organic Solvent Midea”. inBiocatalysis in Organic Media, Laane, C., et al., eds., pp. 21–41, Elsevier, Amsterdam.

    Google Scholar 

  • Freeman, A., and Lilly, M. D. (1987).Appl. Microbiol. Biotechnol.,25, 495–501.

    Google Scholar 

  • Granot, I., Aharonowitz, Y. and Freeman, A. (1987).Appl. Microbiol. Biotechnol.,27, 457–463.

    Google Scholar 

  • Leonhardt, A., Szwajcer, E., Mosbach, K. (1985).Appl. Microbiol. Biotechnol.,21, 162–166.

    Google Scholar 

  • Sonomoto, K., Jin, I. N., Tanaka, A., and Fukui, S. (1980).Agric. Biol. Chem.,44, 1119–1126.

    Google Scholar 

  • Park, T. G. and Hoffman, A. S. (1988). Submitted toBiotech. Bioeng.

  • Tanaka, K. (1981).Sci. Amer. 224, 110–123.

    Google Scholar 

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Authors and Affiliations

  1. Center for Bioengineering, Dept. of Chemical Engineering, University of Washington, 98195, Seattle, WA

    Tae Gwan Park & Allan S. Hoffman

  2. Washington Technology Center, FL-20, University of Washington, 98195, Seattle, WA

    Tae Gwan Park & Allan S. Hoffman

Authors
  1. Tae Gwan Park
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  2. Allan S. Hoffman
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Park, T.G., Hoffman, A.S. Immobilization ofArthrobacter simplex cells in thermally reversible hydrogels: Comparative effects of organic solvent and polymeric surfactant on steroid conversion. Biotechnol Lett 11, 17–22 (1989). https://doi.org/10.1007/BF01026779

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  • DOI: https://doi.org/10.1007/BF01026779

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