Skip to main content
SpringerLink
Log in

Construction ofEscherichia coli-Clostridium acetobutylicum shuttle vectors and transformation ofClostridium acetobutylicum strains

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

SeveralE. coli-C. acetobutylicum shuttle vectors were constructed and used to transform twoC. acetobutylicum strains ATCC 824 and NCIMB 8052. Other than pSYL2, none of these vectors were able to transform ATCC 824 due to the presence of a restriction system. However, all of them could transform NCIMB 8052 with efficiencies of 8×102–6×103 transformants per μg DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Achen, M.G., Davidson, B.E., and Hillier, A.J. (1986).Gene 45, 45–49.

    Google Scholar 

  • Birnboim, H.C., and Doly, J. (1979).Nucleic Acids Res. 7, 1513–1523.

    Google Scholar 

  • Cary, J.W., Petersen, D.J., Papoutsakis, E.T., and Bennett, G.N. (1988),J. Bacteriol. 170, 4613–4618.

    Google Scholar 

  • Clewell, D.B. (1981).Microbiol. Rev. 45, 409–436.

    Google Scholar 

  • Dower, W.J., Miller, J.F., and Ragsdale, C.W. (1988).Nucleic Acids Res. 16, 6127–6145.

    Google Scholar 

  • Hanahan, D. (1985). In:DNA cloning: a practical approach, Glover, D. M., ed. pp. 109–135, Washington, D.C.: IRL Press.

    Google Scholar 

  • Hill, L.R. (1966).J. Gen. Microbiol. 44, 419–437.

    Google Scholar 

  • Jones, D.T., and Woods, D.R. (1986)Microbiol. Rev. 50, 484–524.

    Google Scholar 

  • Lee, S.Y., Mermelstein, L.D., Bennett, G.N., and Papoutsakis, E.T. (1992).Ann. NY Acad. Sci., in press.

  • Luczak, H., Schwarzmoser, H., and Staudenbauer, W.L. (1985).Appl. Microbiol. Biotechnol. 23., 114–122.

    Google Scholar 

  • Macrina, F.L., Evans, R.P., Tobian, J.A., Hartley, D.L., Clewell, D.B., and Jones, K.R. (1983).Gene 25, 145–150.

    Google Scholar 

  • Mahler, I., and Halvorson, H.O. (1980).J. Gen. Microbiol. 120, 259–263.

    Google Scholar 

  • Mermelstein, L.D., Welker, N.E., Bennett, G.N., and Papoutsakis, E.T. (1992).Bio/Technol. 10, 190–195.

    Google Scholar 

  • Richards, D.F., Linnett, P.E., Oultram, J.D., and Young, M. (1988).J. Gen. Microbiol. 134, 3151–3157.

    Google Scholar 

  • Roberts, I., Holmes, W.M., and Hylemon, P.B. (1986).Appl. Environ. Microbiol. 52, 197–199.

    Google Scholar 

  • Simon, D., and Chopin, A. (1988).Biochimie 70, 559–566.

    Google Scholar 

  • Squires, C.H., Heefner, D.L., Evans, R.J., Kopp, B.J., and Yarus, M.J. (1984).J. Bacteriol. 159, 465–471.

    Google Scholar 

  • Truffaut, N., Hubert, J., and Reysset, G. (1989).FEMS Microbiol. Lett. 58, 15–20.

    Google Scholar 

  • Young, M., Minton, N.P., and Staudenbauer, W.L. (1989).FEMS Microbiol. Rev. 63, 301–326.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 305-701, Taejon, Korea

    Sang Yup Lee

  2. Department of Biochemistry and Cell Biology, Rice University, 77251, Houston, TX, USA

    George N. Bennett

  3. Department of Chemical Engineering, Northwestern University, 60208, Evanston, IL, USA

    Eleftherios Terry Papoutsakis

Authors
  1. Sang Yup Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. George N. Bennett
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eleftherios Terry Papoutsakis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, S.Y., Bennett, G.N. & Papoutsakis, E.T. Construction ofEscherichia coli-Clostridium acetobutylicum shuttle vectors and transformation ofClostridium acetobutylicum strains. Biotechnol Lett 14, 427–432 (1992). https://doi.org/10.1007/BF01021259

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01021259

Keywords

Search

Navigation