Abstract
The metabolic characteristics of the penumbral region were examined in spontaneously hypertensive rats one hour after permanent middle cerebral artery occlusion. The effect of hyperglycemia on this region was examined by providing a glucose load prior to occlusion. The depressed concentrations of adenosine triphosphate and elevated levels of lactate in the penumbral region were similar to those found in the ischemic focus. The purported neuroprotective effect of hyperglycemia in the penumbral region was not reflected in an increased high-energy phosphate level in the penumbral region. The rapid deterioration of the metabolic status of this region in this strain of rat suggests that the increased consistency of infarction may come at the expense of the penumbral region, and thus this model may not be well suited for the study of metabolic changes and perhaps even therapeutic intervention.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Astrup J., Symon L., Branston M., Lassen N.A.(1977). Cortical evoked potential and extracellular K+ and H+ at critical levels of brain ischemia.Stroke 8:51–57.
Brint S., Jacewicz M., Liessling M., Tanabe J., Pulsinelli W.(1988). Focal brain ischemia in the rat: Methods for reproducible neocortical infarction using tandem occlusion of the distal middle cerebral and ipsilateral common carotid arteries.J Cereb Blood Flow Met 8:474–485.
Busto R., Ginsberg M.D.(1985). Graded focal cerebral ischemia in the rat by unilateral carotid artery occlusion and elevated intracranial pressure: Hemodynamic and biochemical characterization.Stroke 16:446–476.
Coyle P.(1986). Different susceptibilities to cerebral infarction in spontaneously hypertensive (SHR) and normotensive Sprague-Dawley rats.Stroke 17:520–525.
Duverger D., MacKenzie E.T.(1988). The quantification of cerebral infarction following focal ischemia in the rat: Influence of strain, arterial pressure, blood glucose concentration, and age.J Cereb Blood Flow Metab 8:449–461.
Ginsberg M.D., Prado R., Dietrich W.D., Busto R., Watson B.D.(1987). Hyperglycemia reduces the extent of cerebral infarction in rats.Stroke 18: 570–574.
Grabowski M., Nordborg C., Brundin P., Johansson B.B.(1988). Middle cerebral artery occlusion in the hypertensive and normotensive rat: A study of histopathology and behavior.J of Hypertension 6:405–411.
Lust WD, Ricci AJ, Selman WR, Ratcheson RA: Methods of fixation of nervous tissue for use in the study of cerebral energy metabolism inNeuromethods. Boulton AA, Baker GB, Butterworth RF (eds). Humana Press, New Jersey, 1987. Vol 11, pp 115–149.
Nedergaard M., Diemer N.H.(1986). Focal ischemia of the rat brain. With special reference to plasma glucose concentration.Acta Neuropathol 73:131–137.
Ponten U., Ratcheson, R.A., Salford L.G., Siesjö B.K.(1973) Optimal freezing conditions for cerebral metabolites in rats.J Neurochem 21:1127–1138.
Selman W.R., VanDer Veer C., Whittingham T.S., LaManna J.C., Lust W.D., Ratcheson R.A.(1987). Visually defined zones of focal ischemia in the rat brain.Neurosurgery 21:825–830.
Selman W.R., Ricci A.J., Crumrine R.C., LaManna J.C., Ratcheson R.A., Lust W.D.(1990). The evolution of focal ischemic damage: A metabolic analysis.Metabolic Brain Disease 5:33–44.
Siesjö B.K.(1981). Cell damage in the brain: A speculative synthesis.J Cereb Blood Flow Metab 1:155–185.
Snedecor G.W. and Cochran W.G.(1980). Statistical Methods. The Iowa University Press, Ames, Iowa, U.S.A., Seventh Edition, pp 283–285.
Tamura A., Graham D.I., McCullouch J., Teasdale G.M. (1981). Description of technique and early neuropathological consequences following middle cerebral artery occlusion.J Cereb Blood Flow Metab 1:53–60.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Selman, W.R., Crumrine, R.C., Rosenstein, C.C. et al. Rapid metabolic failure in spontaneously hypertensive rats after middle cerebral artery ligation. Metabolic Brain Disease 6, 57–64 (1991). https://doi.org/10.1007/BF00999903
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00999903