Abstract
The myelin specific proteins, myelin basic protein (MBP) and myelin proteolipid protein (PLP) were quantitated by radioimmunoassay (RIA) and the activity of the enzyme 2′3′-cyclic 3′ phosphohydrolase (CNP) measured, in 27 regions of normal brain and spinal cord. Varying regional concentrations for each protein and regional variations for protein ratios were noted, supporting the concept of a varying chemical composition for myelin throughout the central nervous system (CNS). Variation was also noted among myelin subfractions from a single region. Regions with special sensitivity to the multiple sclerosis process had relatively lower proportions of CNP in several, but not all cases.
Similar content being viewed by others
References
Hartman, B. K., Agrawal, H. C., Agrawal, D., andKalmbach, S. 1982. Development and maturation of central nervous system myelin: Comparison of immunohistochemical localization of proteolipid protein and basic protein in myelin and oligodendrocytes. Proc. Natl. Acad. Sci. 79:4218–4220.
Fujimoto, K., Roots, B. I., Burton, R. M., andAgrawal, H. C. 1976. Morphological and biochemical characterization of light and heavy myelin isolated from developing rat brain. Biochem. Biophys. Acta, 426:659–668.
Zgorzalewicz, B., Neuhoff, V., andWaehneldt, T. V. 1974. Rat myelin proteins. Compositional changes in various regions of the nervous system during ontogenetic development. Neurobiology, 4:265–276.
Zimmerman, A. W., Quarles, R. H., andWebster, H. deF., andMatthieu, J. M., andBrady, R. O. 1975. Characterization and protein analysis of myelin subfractions in rat brain: Developmental and regional comparisons. J. Neurochem. 25:749–757.
Hartman, B. K., Agrawal, H. C., Kalmbach, S., andShearer, W. T. 1979. A comparative study of the immunohistochemical localization of basic protein to myelin and oligodendrocytes in rat and chicken brain. J. Comp. Neurol. 188:273–290.
Mendel, J. R., andWhitaker, J. N. 1978. Immunocytochemical studies of myelin basic protein. J. Cell. Biol. 76:502–511.
Sternberger, N. H., Itoyama, Y., Kies, M. W., andWebster, H. deF. 1978. Myelin basic protein demonstrated immunocytochemically in oligodendroglia prior to myelin sheath formation. Proc. Natl. Acad. Sci. 75:2521–2524.
Fishman, M. A., Agrawal, H. C., Alexander, A., Golterman, J., Martenson, R. E., andMitchell, R. F. 1975. Biochemical maturation of human central nervous system myelin. J. Neurochem. 24:689–694.
Kurihara, T., andTsukada, Y. 1967. The regional and subcellular distribution of 2′3′-cyclic nucleotide 3′ phosphohydrolase in the central nervous system. J. Neurochem. 14:1167–1174.
Sprinkle, T. J., Zaruba, M. E., andMcKhann, G. M. 1978. Activity of 2′3′ cyclic nucleotide 3′ phosphodiesterase in regions of rat brain during development: Quantitative relationship to myelin basic protein. J. Neurochem. 30:309–314.
Toews, A. D., andHorrocks, L. A. 1976. Developmental and aging changes in protein concentration and 2′3′ cyclic nucleotide monophosphate phosphodiesterase activity in human cerebral white and gray matter and spinal cord. J. Neurochem. 27:545–550.
Poduslo, S. E. andNorton, W. T. 1972. Isolation and some chemical properties of oligodendroglia from calf brains. J. Neurochem. 19:727–736.
Agrawal, H. C., Hartman, B. K., Shearer, W. T., Kalmbach, S., andMargolis, F. 1977. Purification and immunohistochemical localization of rat brain myelin proteolipid protein. J. Neurochem. 28:495–508.
Trotter, J. L., Lieberman, L., Margolis, F. L., andAgrawal, H. C. 1981. Radioimmunoassay for central nervous system myelin-specific proteolipid protein. J. Neurochem. 36:1256–1262.
Einstein, E. R., Robertson, D. M., DiCaprio, J. M., andMoore, W. 1962. The isolation from bovine spinal cord of a homogeneous protein with encephalitogenic activity. J. Neurochem. 9:351–361.
Kies, M. W., Murphy, J. B., andAlvord, E. C. 1961. Studies of the encephalitogenic factor in guinea pig central nervous system, pages 197–204,in Folch, P. J. (ed.), Chemical Pathology of the Nervous System, Pergamon Press, Oxford.
Cohen, S. R., McKhann, G. M., andGuarnieri, M. 1975. A radioimmunoassay for myelin basic protein and its use for quantitative measurements. J. Neurochem. 25:371–376.
Palfreyman, J. W., Thomas, D. G. T., andRatcliffe, J. G. 1978. Radioimmunoassay of human myelin basic protein in tissue extract, cerebrospinal fluid and serum and its clinical application to patients with head injury. Clin. Chim. Acta. 82:259–270.
Schmid, G., Thomas, G., Hempel, K., andGruninger, W. 1974. Radioimmunological determination of myelin basic protein (MBP) and MBP-antibodies. Europ. Neurol. 12:173–185.
Agrawal, H. C., Trotter, J. L., Burton, R. M., andMitchell, R. F. 1974. Metabolic studies on myelin. Evidence for a precursor role of a myelin subfraction. Biochem. J. 140:99–109.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurements with the Folin-phenol reagent. J. Biol. Chem. 193:265–225.
Kurihara, T., Nussbaum, J. L., andMandel, P. 1969. 2′3′ cyclic nucleotide 3′ phosphohydrolase in the brain of the “Jimpy” mouse, a mutant with deficient myelination. Brain. Res. 13:401–403.
Deibler, G. E., Martenson, R. E., andKies, M. W. 1972. Large scale preparation of myelin basic protein from central nervous system of several mammalian species. Prep. Biochem. 2:139–165.
Agrawal, H. C. 1974. Analysis of membrane proteins by sodium dodecyl-sulfate-polacrylamide gel electrophoresis, in Fundamentals of Lipid Chemistry. (Burton, R. M., and Guerra, F. C., eds.), pp. 511–543. Bio-Science Publications Division, Webster Groves, Missouri.
Prohaska, J. R., Clark, D. A., andWells, W. W. 1973. Improved rapidity and precision in the determination of brain 2′3′-cyclic nucleotide 3′ phosphydrolase. Anal. Biochem. 56:275–282.
Ansari, K. A., Rand, A., andLoch, J. A. 1978. Biochemical and immunological studies with human optic and olfactory tracts. J. Neuropath. Exp. Neurol. 37:756–767.
Mehl, E., andWolfgram, F. 1969. Myelin types with different protein components in the same species. J. Neurochem. 16:1091–1097.
Amaducci, L., Pazzagli, A., andPessina, G. 1962. The relation of proteolipids and phosphatido-peptides to tissue elements in the bovine nervous system. J. Neurochem. 9:509–518.
Marks, N., Grynbaum, A., andLajtha, A. 1976. The breakdown of myelin-bound proteins by intra- and extracellular proteases. Neurochem. Res. 1:93–111.
Smith, M. E. 1977. The role of proteolytic enzymes in demyelination in experimental allergic encephalomyelitis. Neurochem. Res. 2:233–246.
Fishman, M. A., Trotter, J. L., andAgrawal, H. C. 1977. Selective loss of myelin proteins during autolysis. Neurochem. Res. 2:247–257.
Sammeck, R., andBrady, R. O. 1972. Studies of the catabolism of myelin basic proteins of the rat in situ and in vitro. Brain Res. 42:441–453.
Matthieu, J. M., Koellreutter, B., andJoyet, M. L. 1977. Changes in CNS myelin proteins and glycoproteins after in situ autolysis. Brain Res. Bull. 2:15–21.
Ansari, K. A., Hendrikson, H., Sinha, A. A., andRand, A. T. 1975. Myelin basic protein in frozen bovine brain. A study of autolytic change in situ. J. Neurochem. 25:193–195.
Rand, A., Ansari, K. A., andLock, J. 1979. 2′3′-cyclic nucleotide 3′ phosphohydrolase activity of human white matter and time interval between death and autopsy. J. Neurochem. 32:627–628.
Waehneldt, T. V. 1975. Ontogenetic study of a myelin-derived fraction with 2′3′-cyclic nucleotide-3′-phosphohydrolase activity higher than that of myelin. Biochem. J. 151:435–437.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Trotter, J.L., Wegescheide, C.L. & Garvey, W.F. Regional studies of myelin proteins in human brain and spinal cord. Neurochem Res 9, 133–146 (1984). https://doi.org/10.1007/BF00967665
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00967665