Abstract
Acremonium sect.Albo-lanosa (Fungi Imperfecti) includes beneficial, endophytic mycosymbionts of various grasses of the subfamilyPooideae, and also the anamorph of the grass choke pathogen,Epichloë typhina (Clavicipitaceae, Ascomycotina). These fungi are seed-disseminated, thus stably maintained for many host generations. To investigate the possibility of long-term coevolution, isolates ofE. typhina and anamorphs were obtained from eight grass species, sequences of their rRNA gene internal transcribed spacers were aligned with those from otherClavicipitaceae, and cladograms were generated by maximum parsimony. The results indicated that the nonpathogenic endophytes have not necessarily coevolved with their host species and that they arose fromE. typhina on multiple occasions.
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References
Arachevaleta, M., Bacon, W. C., Hoveland, C. S., Radcliffe, D. E., 1989: Effect of the tall fescue endophyte on plant response to environmental stress. — Agronomy J.81: 83–90.
Abrahams, J. P., van den Berg, M., van Batenburg, E., Pleu, C., 1990: Prediction of RNA secondary structure, including pseudoknotting, by computer simulation. — Nucleic Acids Res.18: 3035–3044.
Bacon, C. W., De Battista, J., 1990: Endophytic fungi of grasses. — InAvora, D. K., Rai, B., Mukerji, K. G., Knudsen, G. R., (Eds.): Soil and plants1, pp. 231–256. — New York, Basel: Marcel Dekker.
Bruns, T. D., Fogel, R., Taylor, J. W., 1990: Amplification and sequencing of DNA from fungal herbarium specimens. — Mycologia82: 175–184.
Byrd, A. D., Schardl, C. L., Songlin, P. J., Mogen, K. L., Siegel, M. R., 1990: The β-tubulin gene ofEpichloë typhina from perennial ryegrass (Lolium perenne). — Curr. Genet.18: 347–354.
Chandrasekharan, P., Lewis, E. J., Borrill, M., 1972: Studies inFestuca. II. Fertility relationships between species of sectionsBovinae andScariosae, and their affinities withLolium. — Genetica43: 375–386.
Clay, K., 1988: Clavicipitaceous fungal endophytes of grasses: coevolution and the change from parasitism to mutualism. — InPirozynski, K. A., Hawksworth, D., (Eds.): Coevolution of fungi with plants and animals, pp. 79–105. — London: Academic Press.
—, 1989: Clavicipitaceous endophytes of grasses: their potential as biocontrol agents. — Mycol. Res.92: 1–12.
—, 1989: Effect of ergot alkaloids from fungal endophyte-infected grasses on fall armyworm (Spodoptera frugiperda). — J. Chem. Ecol.15: 169–182.
Dahlman, D. L., Eichenseer, H., Siegel, M. R., 1991: Chemical perspectives on endophyte-grass interactions and their implications to insect herbivory. — InBarbosa, P., Kirschik, L., Jones, E., (Eds.): Microbial mediation of plant-herbivore interactions, pp. 227–252. — New York: Wiley.
Diehl, W. W., 1950:Balansia and theBalansiae in America. Agricultural Monograph. 4. — Washington, D.C.: United States Department of Agriculture.
Felsenstein, J., 1985: Confidence limits on phylogenies: An approach using the bootstrap. — Evolution39: 783–791.
Free, S. J., Rice, P. W., Metzenberg, R. L., 1979: Arrangement of the genes coding for ribosomal ribonucleic acids inNeurospora crassa. — J. Bacteriol.137: 1219–1226.
Hijwegen, T., 1988: Coevolution of flowering plants with pathogenic fungi. — InPirozynski, K. A., Hawksworth, D. L., (Eds.): Coevolution of fungi with plants and animals, pp. 63–77. — London: Academic Press.
Hitchcock, A. S., 1950: Manual of the grasses of the United States. Publication no. 200. — Washington, D.C.: United States Department of Agriculture.
Johnson, M. C., Pirone, T. P., Siegel, M. R., Varney, D. R., 1982: Detection ofEpichloë typhina in tall fescue by means of enzyme-linked immunosorbent assay. — Phytopathology72: 647–650.
Jorgensen, R. A., Cluster, P. D., 1988: Modes and tempos in the evolution of nuclear ribosomal DNA: New characters for evolutionary studies and new markers for genetic and population studies. — Ann. Missouri Bot. Gard.75: 1238–1247.
Kimmons, C. A., Gwinn, K. D., Bernard, E. C., 1990: Nematode reproduction on endophyte-infected and endophyte-free tall fescue. — Pl. Dis.74: 757–761.
Latch, G. C. M., Christensen, M. J., 1988: Endophytes of annual and hybrid ryegrasses. — New Zealand J. Agr. Res.31: 57–63.
Law, R., Lewis, D. H., 1983: Biotic environments and the maintenance of sex—some evidence from mutualistic symbioses. — Biol. J. Linn. Soc.20: 249–276.
Leuchtmann, A., Clay, K., 1990: Isozyme variation in theAcremonium/Epichloe fungal endophyte complex. — Phytopathology80: 1133–1139.
Li, W.-H., Gouy, M., 1990: Statistical tests of molecular phylogenies. — Methods Enzymol.183: 645–659.
Muller, H. J., 1964: The relation of recombination to mutational advance. — Mutat. Res.1: 2–9.
Powell, R. G., Plattner, R. D., Yates, S. G., Clay, K., Leuchtmann, A., 1990: Ergobalansine, a new ergot-type peptide alkaloid isolated fromCenchrus echinatus (sandbur grass) infected withBalansia obtecta, and produced in liquid cultures ofB. obtecta andBalansia cyperi. — J. Nat. Products, Lloydia53: 1272–1279.
Prestidge, R. A., di Menna, M. E., Van der Zijpp, S., 1985: MinimumAcremonium loliae levels needed to maintain argentine stem weevil resistance in infected ryegrasses and in cultures. Proc. 4th Australasian Conf. on Grassl. Invert. Ecol., pp. 332–337. — Lincoln College, Canterbury: Caxton Press.
Qu, L.-H., Nicoloso, M., Bachellerie, J.-P., 1988: Phylogenetic calibration of the 5′ terminal domain of large rRNA achieved by determining twenty eucaryotic sequences. — J. Mol. Evol.28: 113–124.
Schardl, C. L., Siegel, M. R., 1991: Molecular genetics ofAcremonium coenophialum andEpichloë typhina. — Agr. Ecosystems Environ. (in press).
Siegel, M. R., Schardl, C. L., 1991: Fungal endophytes of grasses: Detrimental and beneficial associations. — InAndrew, J. H., Hirano, S. S., (Eds.): Microbial ecology of leaves. — Berlin: Springer (in press).
—, 1990: Fungal endophyte-infected grasses: Alkaloid accumulation and aphid response. — J. Chem. Ecol.16: 3301–3315.
Sun, S., Clarke, B., Funk, C. R., 1990: Effect of fertilizer and fungicide applications on choke expression and endophyte transmission in chewings fescue. — InQuisenberry, S. S., Joost, R. E., (Eds.): Proc. Int. Symp.Acremonium/Grass Interactions, New Orleans, pp. 62–63. — Baton Rouge, Louisiana: Louisiana Agricultural Experiment Station.
Stuedemann, J. A., Hoveland, C. S., 1988: Fescue endophyte: history and impact on animal agriculture. — J. Prod. Agric.1: 39–44.
Swofford, D. L., 1990: PAUP: phylogenetic analysis using parsimony, Version 3.0q. — Champaign, Illinois: Illinois Natural History Survey.
Toneguzzo, F., Glynn, S., Levi, E., Mholsness, S., Hayday, A., 1988: Use of a chemically modified T 7 DNA polymerase for manual and automated sequencing of supercoiled DNA. — Biotechniques6: 460–469.
White, J. F., Jr., 1987: Widespread distribution of endophytes in thePoaceae. — Pl. Dis.71: 340–342.
—, 1988: Endophyte-host associations in forage grasses. IX. A proposal concerning origin and evolution. — Mycologia80: 442–446.
—, 1987: Endophyte-host associations in forage grasses. VIII. Heterothallism inEpichloë typhina. — Amer. J. Bot.74: 1716–1721.
White, T. J., Bruns, T. D., Lee, S., Taylor, J., 1990: Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. — InInnis, M. A., Gelfand, D. H., Sninsky, J. J., White, T. J., (Eds.): PCR Protocols: A guide to methods and applications, pp. 315–322. — San Diego, California: Academic Press.
Yeh, L.-C. C., Lee, J. C., 1990: Structure analysis of the internal transcribed spacer 2 of the precursor ribosomal RNA fromSaccharomyces cerevisiae. — J. Mol. Biol.211: 699–712.
—, 1990: Internal transcribed spacer-1 of the yeast precursor ribosomal RNA—Higher order structure and common structural motifs. — Biochemistry29: 5911–5918.
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Schardl, C.L., Liu, JS., White, J.F. et al. Molecular phylogenetic relationships of nonpathogenic grass mycosymbionts and clavicipitaceous plant pathogens. Pl Syst Evol 178, 27–41 (1991). https://doi.org/10.1007/BF00937980
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DOI: https://doi.org/10.1007/BF00937980