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Determination of sequence and linkage of tissue oligosaccharides in caprine β-mannosidosis by fast atom bombardment, collisionally activated dissociation tandem mass spectrometry

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Abstract

Fast atom bombardment, collisionally activated dissociation tandem mass spectrometry (FAB-CAD-MS/MS), combined withp-aminobenzoic acid ethyl ester (ABEE) derivatization, were used to confirm the sequence and linkage pattern of subnanomolar amounts of the previously characterized three major thyroid gland oligosaccharides accumulated in caprine β-mannosidosis. Positive ion FAB-CAD-MS/MS of both the [M + H]+ and [M + Na]+ ions from the ABEE derivatized oligosaccharides produced product ions derived from cleavage of the glycosidic bonds which allowed the sequences to be determined. Several fragments resulting from cleavages across the sugar ring permitted the assignment, in some cases, of the linkage positions between the sugar residues. The natriated molecule yielded several fragments of this type which were not observed when the protonated molecule was selected as the precursor ion. Use of these techniques gave the complete sequence and linkage characterization of the disaccharide and complete sequence and partial linkage information for the two higher oligosaccharides.

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Authors and Affiliations

  1. MSU-NIH Mass Spectrometry Facility, Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA

    Douglas A. Gage

  2. Department of Pathology, Michigan State University, 48824, East Lansing, MI, USA

    Eileen Rathke & Margaret Z. Jones

  3. NIH Mass Spectrometry Facility, Department of Chemistry, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA

    Catherine E. Costello

Authors
  1. Douglas A. Gage
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  2. Eileen Rathke
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  3. Catherine E. Costello
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  4. Margaret Z. Jones
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Gage, D.A., Rathke, E., Costello, C.E. et al. Determination of sequence and linkage of tissue oligosaccharides in caprine β-mannosidosis by fast atom bombardment, collisionally activated dissociation tandem mass spectrometry. Glycoconjugate J 9, 126–131 (1992). https://doi.org/10.1007/BF00780759

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  • DOI: https://doi.org/10.1007/BF00780759

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