Summary
The Golgi-Cox method was used to compare transverse sections of the lumbar cords of amyotrophic lateral sclerosis (ALS) patients and controls. Large anterior horn cells of the control cords could be divided into three groups based on arrangement of the dendrites arising from the soma; bipolar, tripolar and multipolar. Axons could be identified by their characteristic morphology; tapering axon hillocks and initial segments, followed by an increase in caliber at the first myelinated segments. Axons usually emanated from somata, but sometimes from dendrites. Only a single abnormal neuron with a plump soma and very thin dendrites, was seen in the controls. Atrophic neurons were rare in the controls. In ALS, various abnormal changes were found in the anterior horn cells. In some, the axon remained thin and did not attain a normal thickness at the point where one might expect the first myelinated segment. The normal morphology of the dendrites was sometimes disturbed resulting in a poor extension of the dendritic trees, and thin dendrites. This communication is the first description of the Golgi method applied to ALS cases.
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Abdel-Maguid TE, Bowsher D (1984) Classification of neurons by dendritic branching pattern. A categorisation based on Golgi impregnation of spinal and cranial somatic and visceral afferent and efferent cells in the adult human. J Anat 138:689–702
Carpenter S (1968) Proximal axonal enlargement in motor neuron disease. Neurology 18:841–851
Conradi S (1969) Observations on the ultrastructure of the axon hillock and initial axon segment of lumbosacral motoneurons in the cat. Acta Physiol Scand [Suppl] 332: 65–84
Ferrer I (1984) A Golgi analysis of unlayered polymicrogyria. Acta Neuropathol (Berl) 65:69–76
Fujisawa K, Nakamura A (1982) The human Purkinje cells: a Golgi study in pathology. Acta Neuropathol (Berl) 56:255–264
Hirano A, Donnenfeld H, Sasaki S, Nakano I (1984) Fine structural observations of neurofilamentous changes in amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 43:461–470
Hirano A, Nakano I, Kurland LT, Mulder DW, Holley PW, Saccomanno G (1984) Fine structural study of neurofibrillary changes in a family with amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 43:471–480
Inoue K, Hirano A (1979) Early pathological changes of amyotrophic lateral sclerosis. Autopsy findings of a case of 10 month's duration. Neurol Med Chir (Tokyo) 11:448–455
Inoue K, Hirano A (1980) Early pathological changes of amyotrophic lateral sclerosis: a reappraisal of the spheroid, Bunina body and morphometry of the ventral spinal root. J Neuropathol Exp Neurol 39:363 (abstr)
Kato T, Hirano A (1985) A Golgi study of the proximal portion of the human Purkinje cell axon. Acta Neuropathol (Berl) 68:191–195
Kato T, Hirano A (1986) A collateral branch arising from the initial segment of the axon of an anterior horn cell. Neurol Med Chir (Tokyo) 25:310–311
Kato T, Hirano A, Llena JF (1985) A Golgi study of the human Purkinje cell soma and dendrites. Acta Neuropathol (Berl) 68:145–148
Landis DMD, Williams RS, Masters CL (1981) Golgi and electron-microscopic studies of spongiform encephalopathy. Neurology 31:538–549
Marin-Padilla M (1976) Pyramidal cell abnormalities in the motor cortex of a child with Down's syndrome. A Golgi study. J Comp Neurol 167:63–82
Nakamura S, Akiguchi I, Kameyama M, Mizuno N (1985) Age-related changes of pyramidal cell basal dendrites in layers III and V of human motor cortex: a quantitative Golgi study. Acta Neuropathol (Berl) 65:281–284
Nakano I, Hirano A (1983) Morphology of processes of large lower motor neurons in the human spinal cord: observation with modified Bielschowsky's silver impregnation method for the axon. Neurol Med Chir (Tokyo) 18:567–574
Nakano I, Hirano A (1987) Atrophic cell processes of large motor neurons in the anterior horn in amyotrophic lateral sclerosis: observation with silver impregnation method. J Neuropathol Exp Neurol 46:40–49
Nakano I, Hirano A, Kurland LT, Mulder DW, Holley PW, Saccomanno G (1984) Familial amyotrophic lateral sclerosis: Neuropathology of two brothers in American “C” family. Neurol Med Chir (Tokyo) 20:458–471
Purpura DP, Baker HJ (1977) Meganeurites and other aberrant processes of neurons in feline GM1-gangliosidosis: a Golgi study. Brain Res 143:13–26
Purpura DP, Suzuki K (1976) Distortion of neuronal geometry and formation of aberrant synapses in neuronal storage disease. Brain Res 116:1–12
Purpura DP, Hirano A, French JH (1976) Polydendritic Purkinje cells in X-chromosome linked copper malabsorption: a Golgi study. Brain Res 117:125–129
Scheibel AB, Tomiyasu U (1978) Dendritic sprouting in Alzheimer's presenile dementia. Exp Neurol 60:1–8
Scheibel ME, Scheibel AB (1966) Spinal motorneurons, interneurons and Renshaw cells. A Golgi study. Arch Ital Biol 104:328–353
Scheibel ME, Scheibel AB (1975) Structural changes in the aging brain. Aging NY 1:11–37
Schochet SS Jr, Hardman JM, Ladewig PP, Earle KM (1969) Intraneuronal conglomerates in sporadic motor neuron disease. Arch Neurol 20:548–553
Sholl DA (1953) Dendritic organization in the neurons of the visual cortices of the cat. J Anat 87:387–407
Udaka F, Kameyama M, Tomonaga M (1986) Degeneration of Betz cells in motor neuron disease. A Golgi study. Acta Neuropathol (Berl) 70:289–295
Walkley SU, Blakemore WF, Purpura DP (1981) Alterations in neuron morphology in feline mannosidosis. A Golgi study. Acta Neuropathol (Berl) 53:75–79
Westrum LE (1970) Observation on initial segments of axons in the prepyriform cortex of the rat. J Comp Neurol 139:337–356
Williams RS, Ferrante RJ, Caviness VS Jr (1978) The Golgi rapid method in clinical neuropathology: the morphologic consequences of suboptimal fixation. J Neuropathol Exp Neurol 37:13–33
Williams RS, Marshall PC, Lott IT, Caviness VS Jr (1978) The cellular pathology of Menkes steely hair syndrome. Neurology 28:575–583
Yamano T, Suzuki K (1985) Abnormalities of Purkinje cell arborization in brindled mouse cerebellum. A Golgi study. J Neuropathol Exp Neurol 44:85–96
Yoshioka H, Yoshida A, Ochi M, Ilino S, Takeuchi Y, Kusunoki T (1986) Dendritic development of cortical neurons of mice subjected to total asphyxia: a Golgi study. Acta Neuropathol (Berl) 70:185–189
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Kato, T., Hirano, A. & Donnenfeld, H. A golgi study of the large anterior horn cells of the lumbar cords in normal spinal cords and in amyotrophic lateral sclerosis. Acta Neuropathol 75, 34–40 (1987). https://doi.org/10.1007/BF00686790
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DOI: https://doi.org/10.1007/BF00686790