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The effect of furosemide on luminal sodium, chloride and potassium transport in the early distal tubule ofAmphiuma kidney

Effects of potassium adaptation

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
  • Published:
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Abstract

Previous experiments in the early distal tubule of the doubly perfused kidney ofAmphiuma demonstrated net reabsorption of potassium (K) which is reversed to net K secretion after K adaptation. Furthermore, it is known that this particular segment exhibits extensive chloride (Cl) net reabsorption which depends on the presence of sodium (Na) and which is inhibited by furosemide. In order to test for a possible interrelationship between NaCl and K transport, K activity in lumen and cell, transepithelial electrical potential difference, peritubular cell membrane potentials and volume reabsorption were measured in control animals and after K adaptation, in presence and absence of furosemide.

In control animals the direction of net K transport is reversed from reabsorption to secretion upon addition of furosemide or following the removal of Cl from the tubular lumen. Volume reabsorption is inhibited by some 80%. In K adapted animals a similar inhibition of volume reabsorption is observed, however K secretion is not further enhanced. In control as well as in K-adpated animals intracellular K activities are still above electrochemical equilibrium after furosemide.

The data suggest that a common transport system for Na, Cl and K is present in the luminal cell membrane which is inhibited by furosemide, K secretion observed in controls after furosemide and in K-adapted animals is driven by the cell to lumen electrochemical gradient for K across the K permeable luminal cell membrane. The shift of the luminal pump-leak system towards K secretion following K adaptation may be explained by an increase of the luminal K conductance and/or by a reduction of the activity of the luminal cotransport system. However, other mechanisms may also contribute to the observed phenomenon of K adaptation and cannot be ruled out at present.

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References

  1. Burg MB, Bourdeau JE (1977) Function of the thick ascending limb of Henle's loop. In: Vogel HG, Ullrich KJ (eds) New aspects of renal function. Excerpta Medica, Amsterdam Oxford

    Google Scholar 

  2. Burg M, Stoner J, Cardinal J, Green N (1973) Furosemide effect on isolated perfused tubules. Am J Physiol 225:119–124

    Google Scholar 

  3. Finkelstein FO, Hayslett JP (1975) Role of medullary Na-K-ATPase in renal potassium adaptation. Am J Physiol 229:524–528

    Google Scholar 

  4. Fossat B, Lahlou B (1979) The mechanism of coupled transport of sodium and chloride in isolated urinary bladder of the trout. J Physiol 294:211–222

    Google Scholar 

  5. Friedman PA, Andreoli TE (1982) CO2-stimulated NaCl absorption in the mouse renal cortical thick ascending limb of Henle — Evidence for synchronous Na+/H+ and Cl−/HCO −3 exchange in apical plasma membranes. J Gen Physiol (in press)

  6. Frizzell RA, Smith PL, Vosburgh E, Field M (1979) Coupled sodium-chloride influx across brush border of flounder intestine. J Membr Biol 46:27–36

    Google Scholar 

  7. Fujimoto M, Kubota T (1976) Physiochemical properties of a liquid ion exchanger microelectrode and its application to biological fluids. Jpn J Physiol 26:631–650

    Google Scholar 

  8. Gardner JD, Aurbach GD, Spiegel AM, Brown EM (1976) Receptor function and ion transport in turkey erythrocytes. Recent Prog Horm Res 32:567–595

    Google Scholar 

  9. Geck P, Pietrzyk C, Burckhardt BC, Pfeiffer B, Heinz E (1980) Electrically silent cotransport of Na+, K+ and Cl− in Ehrlich cells. Biocheim et Biophys Acta 600:432–447

    Google Scholar 

  10. Gertz K (1962) Direct measurement of the transtubular flux of electrolytes and non-electrolytes in the intact rat kidney. Proc 22nd Intern Congr Physiolog Sciences, Leyden, p 370

  11. Giebisch G (1979) Renal potassium transport. In: Giebisch G, Tosteson D, Ussing HH (eds) Transport across biological membranes. Springer, Berlin Heidelberg New York

    Google Scholar 

  12. Greger R (1981) Chloride reabsorption in the rabbit cortical thick ascending limb of Henle — a sodium dependent process. Pflügers Arch 390:38–43

    Google Scholar 

  13. Greger R, Schlatter E (1981) Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 392:92–94

    Google Scholar 

  14. Greger R, Schlatter E, Lang F (1983) Evidence for electroneutral chloride cotransport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch (in press)

  15. Greger R, Schlatter E (1983) Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch (in press)

  16. Greger R, Schlatter E (1983) Properties of the basolateral membrane of the cortical thick ascending of Henle's loop of rabbit kidney. A model for secondary active chloride transport. Pflügers Arch (in press)

  17. Guggino WB, Stanton BA, Giebisch G (1982) Electrical properties of isolated distal tubule ofAmphiuma. Fed Proc 14:1579

    Google Scholar 

  18. Hansen LL, Schilling AR, Wiederholt M (1981) Effect of calcium, furosemide and chlorothiazide on net volume reabsorption and basolateral membrane potential of the distal tubule. Pflügers Arch 189:121–126

    Google Scholar 

  19. Hebert SC, Culpepper RM, Andreoli TE (1981) NaCl transport in mouse medullary thick ascending limbs. II. ADH enhancement of transcellular NaCl cotransport; origin of transepithelial voltage. Am J Physiol 241:F432-F442

    Google Scholar 

  20. Imai M (1977) Effect of bumetanide and furosemide on the thick ascending limb of Henle's loop of rabbits and rats perfused in vitro. Eur J Pharmacol 41:409–416

    Google Scholar 

  21. Machen TE, McLennan WL (1980) Na+-dependent H+ and Cl− transport in in vitro frog gastric mucosa. Am J Physiol 238:G403-G413

    Google Scholar 

  22. McManus TJ, Schmidt WF III (1978) Ion and co-ion transport in avian red cells. In: Hoffman JF (ed) Membrane transport processes, vol I. Raven Press, New York, pp 97–106

    Google Scholar 

  23. Nellans HN, Frizzell RA, Schultz SG (1973) Coupled sodiumchloride influx across the brush border of rabbit ileum. Am J Physiol 225:467–475

    Google Scholar 

  24. Oberleithner H, Giebisch G (1981) Mechanism of potassium transport across distal tubular epithelium of Amphiuma. In: MacKnight ADC, Leader JP (eds) Epithelial ion and water transport. Raven Press, New York, pp 97–105

    Google Scholar 

  25. Oberleithner H, Guggino W, Giebisch G (1981) The cellular mechanism of potassium adaptation in the distal amphibian nephron. J Physiol 318:55p

    Google Scholar 

  26. Oberleithner H, Giebisch G, Lang F, Wang W (1982) Cellular mechanism of the furosemide sensitive transport system in the kidney. Klin Wochenschr 60:1173–1179

    Google Scholar 

  27. Oberleithner H, Guggino W, Giebisch G (1982) Mechanism of distal tubular chloride transport in Amphiuma kidney. Am J Physiol 242:F331-F339

    Google Scholar 

  28. Oberleithner H, Guggino W, Giebisch G (1983) Mechanism of potassium transport after potassium adaptation in the early distal tubule ofAmphiuma kidney. Pflügers Arch (in press)

  29. Oberleithner H, Lang F, Greger R, Wang W, Giebisch G (1983) Effect of luminal potassium on cellular sodium activity in the early distal tubule ofAmphiuma kidney. Pflügers Arch 396:34–40

    Google Scholar 

  30. Oberleithner H, Lang F, Wang W, Deetjen P (1982) Potassium (K+)-adaptation affects chloride (Cl−) reabsorption in the diluting segment of Amphiuma. Nieren und Hochdruckkrankheiten 5:180

    Google Scholar 

  31. Oberleithner H, Lang F, Wang W, Giebisch G (1982) Effects of inhibition of chloride transport on intracellular sodium activity in distal amphibian nephron. Pflügers Arch 394:55–60

    Google Scholar 

  32. Palfrey HC, Feit PW, Greengard P (1980) cAMP-stimulated cation cotransport in avian erythrocytes: inhibition by “loop” diuretics. Am J Physiol 238:C139-C148

    Google Scholar 

  33. Quamme GA (1981) Effect of furosemide on calcium and magnesium transport in the rat nephron. Am J Physiol 241:F340-F347

    Google Scholar 

  34. Rocha AS, Kokko JP (1973) Sodium chloride and water transport in the medullary thick ascending limb of Henle: Evidence for active chloride transport. J Clin Invest 51:612–623

    Google Scholar 

  35. Sackin H, Morgunov N, Boulpeap EL (1982) Electrical potentials and luminal membrane ion transport in the amphibian renal diluting segment. Fed proc 41 (5):1495

    Google Scholar 

  36. Shindo T, Spring KR (1981) Chloride movement across the basolateral membrane of proximal tubule cells. J Membr Biol 58:35–42

    Google Scholar 

  37. Stanton B, Guggino W, Giebisch G (1981) Electrophysiology of isolated and perfused distal tubules of Amphiuma. 14th Annual Meeting of the American Society of Nephrology, Washington, p 162A

  38. Stoner LC (1977) Isolated perfused amphibian renal tubules: The diluting segment. Am J Physiol 233:F438-F444

    Google Scholar 

  39. Sullivan WJ (1968) Electrical potential differences across distal renal tubules of Amphiuma. Am J Physiol 214:1096–1103

    Google Scholar 

  40. Walker JL (1971) Ionic specific liquid ion exchanger microelectrodes. Anal Chem 43:89A-93A

    Google Scholar 

  41. Wiederholt M, Sullivan WJ, Giebisch G (1971) Potassium and sodium transport across single distal tubules of Amphiuma. J Gen Physiol 57:495–525

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, University of Innsbruck, Fritz-Pregl-Strasse 3, A-6010, Innsbruck, Austria

    H. Oberleithner

  2. Department of Physiology, Yale University, School of Medicine, New Haven, CT, USA

    W. Guggino & G. Giebisch

Authors
  1. H. Oberleithner
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  2. W. Guggino
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  3. G. Giebisch
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Additional information

This work was supported by NIH Grant PHS AM 17633 and AM 29848. Dr. H. Oberleithner received support from Fogarty International Fellowship SF505 TWO 3865-02.

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Oberleithner, H., Guggino, W. & Giebisch, G. The effect of furosemide on luminal sodium, chloride and potassium transport in the early distal tubule ofAmphiuma kidney. Pflugers Arch. 396, 27–33 (1983). https://doi.org/10.1007/BF00584694

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  • DOI: https://doi.org/10.1007/BF00584694

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