Summary
Carbohydrate depletion during exercise was measured in the liver, in the three different types of skeletal muscle, and in the blood of exercise-trained and untrained rats. The acute exercise test consisted of 45 min of treadmill running of progressively increasing intensity. The training program consisted of 6 hrs of swimming per day, 5 days per week for 14 weeks; the training induced an increase of approximately 35% in the respiratory capacity of gastrocnemius muscle, and a 14% increase in heart weight. Glycogen stores in fast-twitch red, fast-twitch white, and slow-twitch red types of skeletal muscle, were depleted significantly more slowly in the trained than in the untrained animals during the treadmill exercise test. Resting glycogen stores in the liver were higher and were depleted more slowly during exercise in the trained than in the untrained animals. Blood lactate concentration was significantly lower in the trained than in the untrained rats at the end of the exercise test. These results provide evidence that endurance exercise training induces adaptations which protect against the depletion of glycogen from the liver and from the three types of skeletal muscle during prolonged exercise.
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References
Ahlborg, B., Bergstrom, J., Ekelund, L. G., Hultman, E.: Muscle glycogen and muscle electrolytes during prolonged physical exercise. Acta physiol. scand.70, 129–142 (1967)
Ariano, M. A., Armstrong, R. B., Edgerton, V. R.: Hind limb muscle fiber populations of five mammals. J. Histochem. Cytochem.21, 51–55 (1973)
Baldwin, K. M., Klinkerfuss, G. H., Terjung, R. L., Mole, P. A., Holloszy, J. O.: Respiratory capacity of white, red and intermediate muscle: adaptive response to exercise. Amer. J. Physiol.222, 373–378 (1972)
Baldwin, K. M., Reitman, J. S., Terjung, R. L., Winder, W. W., Holloszy, J. O.: Substrate depletion in different types of muscle and in liver during prolonged running. Amer. J. Physiol.225, 1045–1050 (1973)
Bergstrom, J., Hermansen, L., Hultman, E., Saltin, B.: Diet, muscle glycogen and physical performance. Acta physiol. scand.71, 140–150 (1967)
Christensen, E. H., Hansen, O.: Respiratorischer Quotient und O2-Aufnahme. Skand. Arch. Physiol.81, 180–189 (1939)
Edgerton, V. R., Simpson, D. R., Barnard, R. J., Peter, J. B.: Phosphorylase activity in acutely exercised muscle. Nature (Lond.)225, 866–867 (1970)
Gollnick, P. D., Armstrong, R. B., Saltin, B., Saubert, C. W., IV, Sembrowich, W. L., Shepherd, R. E.: Effect of training on enzyme activity and fiber composition of human skeletal muscle. J. appl. Physiol.34, 107–111 (1973)
Gollnick, P. D., Piehl, K., Saubert, C. W., IV, Armstrong, R. B., Saltin, B.: Diet, exercise and glycogen changes in human muscle fibers. J. appl. Physiol.33, 421–425 (1972)
Hartley, L. H., Mason, J. W., Hogan, R. P., Jones, L. G., Kotchen, T. A., Mougey, E. H., Wherry, F. E., Pennington, L. L., Ricketts, P. T.: Multiple hormonal responses to prolonged exercise in relation to physical training. J. appl. Physiol.33, 607–610 (1972)
Hassid, W. Z., Abraham, S.: Chemical procedures for analysis of polysaccharides. Methods Enzymol.3, 35–36 (1957)
Henneman, E.: Organization of the spinal cord. In: Medical Physiology, 13th ed., pp. 636–650. Edit. by V. B. Mountcastle, St. Louis: C. V. Mosby 1974
Hermansen, L., Hultman, E., Saltin, B.: Muscle glycogen during prolonged severe exercise. Acta physiol. scand.71, 129–139 (1967)
Hohorst, H. J.: Determination ofl-lactate with LDH and DPN. In: Methods of enzymatic analysis, pp. 266–270, Edit. by H. U. Bergmeyer New York: Academic 1965
Holloszy, J. O.: Biochemical adaptations in muscle. Effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle. J. biol. Chem.242, 2278–2282 (1967)
Holloszy, J. O.: Biochemical adaptations to exercise: aerobic metabolism. In: Exercise and sport sciences reviews, vol. 1, pp. 45–71. Edit. by J. H. Wilmore, New York: Academic 1973
Issekutz, B., Jr., Miller, H. I., Rodahl, K.: Lipid and carbohydrate metabolism during exercise. Fed. Proc.25, 1415–1420 (1966)
Karlsson, J., Nordesjo, L.-P., Saltin, B.: Muscle glycogen utilization during exercise after physical training. Acta physiol. scand.90, 210–217 (1974)
Molé, P., Oscai, L. P., Holloszy, J. O.: Adaptations of muscle to exercise. Increase in the levels of palmityl CoA synthetase, carnitine palmityltransferase and palmityl CoA dehydrogenase, and in the capacity to oxidize fatty acids. J. clin. Invest.50, 2323–2330 (1971)
Morgan, T. E., Cobb, L. A., Short, F. A., Ross, R., Gunn, D. R.: Effects of long-term exercise on human skeletal muscle mitochondria. In: Muscle metabolism during exercise, pp. 87–95. Edit. by B. Pernow and B. Saltin, New York: Plenum 1971
Oscai, L. B., Mole, P. A., Krusack, L. M., Holloszy, J. O.: Detailed body composition analysis on female rats subjected to a program of swimming. J. Nutr.103, 412–418 (1974)
Pruett, E. D. R.: Glucose and insulin during prolonged work stress in men living on different diets. J. appl. Physiol.28, 199–208 (1970)
Rowell, L. B.: The liver as an energy source in man during exercise. In: Muscle metabolism during exercise, pp. 127–141. Edit. by B. Pernow and B. Saltin, New York: Plenum 1971
Rowell, L. B.: Human cardiovascular adjustments to exercise and thermal stress. Physiol. Rev.54, 75–159 (1974)
Saltin, B., Karlsson, J.: Muscle ATP, CP, and lactate during exercise after physical conditioning. In: Muscle metabolism during exercise, pp. 395–399. Edit. by B. Pernow and B. Saltin. New York: Plenum 1971
Slein, M. W.:d-Glucose determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Methods of enzymatic analysis, pp. 117–123. Edit. by H. U. Bergmeyer. New York: Academic 1965.
Terjung, R. L., Baldwin, K. M., Mole, P. A., Klinkerfuss, G. H., Holloszy, J. O.: Effect of running to exhaustion on skeletal muscle mitochondria: a biochemical study. Amer. J. Physiol.223, 549–554 (1972)
Whipp, B. J., Wasserman, K.: Oxygen uptake kinetics for various intensities of constant-load work. J. appl. Physiol.33, 351–356 (1972)
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Baldwin, K.M., Fitts, R.H., Booth, F.W. et al. Depletion of muscle and liver glycogen during exercise. Pflugers Arch. 354, 203–212 (1975). https://doi.org/10.1007/BF00584644
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DOI: https://doi.org/10.1007/BF00584644