Abstract
Frog skin epithelium, which is normally almost tight to chloride, acquires a basolateral leakiness to chloride during osmotic swelling. By measuring the epithelial thickness (volume) after equilibration first with half thiocyanate Ringer, and then full thiocyanate Ringer, one obtains the chloride-free volume. Partial or full recovery of the volume and cellular chloride concentration occurs only when the inside of the skin is exposed to solutions containing K as well as Na and Cl. This recovery process is totally inhibited by low concentrations of bumetanide. The data suggest a basolateral NaKCl2 co-transport.
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Ussing, H.H. Volume regulation and basolateral co-transport of sodium, potassium, and chloride ions in frog skin epithelium. Pflugers Arch. 405 (Suppl 1), S2–S7 (1985). https://doi.org/10.1007/BF00581771
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DOI: https://doi.org/10.1007/BF00581771