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Thermotropism and hydration properties of POPE and POPE-cholesterol systems as revealed by solid state2H and31P-NMR

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Abstract

The partial phase diagram and the hydration properties of the 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE)-water system, in the absence and presence of 30 mol% cholesterol, have been investigated by solid state phosphorus NMR of the lipid and deuterium NMR of heavy water. The POPE-D2O phase diagram resembles other phosphatidylethanolamine (PE)-water systems: below water-to-lipid molar ratios (Ri) of 3 the lamellar gel (Lβ or Lc)-to-hexagonal type II (HII) phase sequence is observed on increasing the temperature. For Ri≥3 the thermotropic sequence (Lβ or Lc)-Lα-HII is detected. On increasing hydration from Ri=3, the HII phase is detected from 40°C to 85°C whereas the gel phase is observed from 40°C to 30°C. The limiting hydrations of the gel, Lα and HII phases are Ri ≈ 3, 17 and 20, respectively. The number of bound water molecules per lipid is ca. 8 in both the La and HII phases. The presence of cholesterol stabilizes the hexagonal phase 20°C below temperatures at which it is observed in its absence and reduces the limiting hydration of the fluid and hexagonal phases to Ri ≈ 9 and 14, respectively. The structure and/or dynamics of the water bound to the interface are markedly modified on going from the Lα to the HII phase.

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Abbreviations

NMR:

Nuclear magnetic resonance

DDPE:

1,2-Didodecyl-rac-glycerol-3-phosphoethanol-amine

DHPE:

1,2-Dihexadecyl-sn-glycerol-3-phosphoethanol-amine

DOPE:

1,2-Dioleoyl-sn-glycerol-3-phosphoethanol-amine

POPE:

1-Palmitoyl-2-oleoyl-sn-glycerol-3-phosphoetha-nolamine

DAPE:

1,2-Diarachinoyl-sn-glycerol-3-phosphoethanol-amine

DMPC:

1,2-Dimyristol-sn-glycerol-3-phosphocholine

DPPC:

1,2-Dipalmitoyl-sn-glycerol-3-phosphocholine

Tc :

lamellar gel-to-lamellar fluid transition temperature

Th :

lamellar fluid-to-hexagonal transition temperature

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Authors and Affiliations

  1. Institute of Solid State Physics, Bulgarian Academy of Sciences, Sofia, Bulgaria

    Radoslav Marinov

  2. Centre de Recherche Paul Pascal, CNRS, F-33600, Pessac, France

    Erick J. Dufourc

Authors
  1. Radoslav Marinov
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  2. Erick J. Dufourc
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Marinov, R., Dufourc, E.J. Thermotropism and hydration properties of POPE and POPE-cholesterol systems as revealed by solid state2H and31P-NMR. Eur Biophys J 24, 423–431 (1996). https://doi.org/10.1007/BF00576714

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  • DOI: https://doi.org/10.1007/BF00576714

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