Abstract
RE6 is a herpes simplex type-1 (HSV-1) x herpex simplex type-2 (HSV-2) intertypic recombinant that cannot replicate in the adult mouse nervous system. In the accompanying report, we have shown that HSV-1 sequences between 0.698 and 0.721 map units can restore a partial neurovirulent phenotype to RE6. In this report, we have used comparative DNA sequence analysis of RE6, 17syn + (HSV-1) and HG52 (HSV-2) to demonstrate that this region contains a site of recombination between HSV-1 and HSV-2 sequences in RE6. High resolution transcription analysis has demonstrated that three readily detected transcripts are present in this region of the genome. In addition, the 5′ end of a low abundance 5 kb transcript was also located in the right-hand portion of this region. All the transcripts encoded by HSV-1 and HSV-2 in this region of the genome are expressed by the RE6 recombinant. This and our sequence data suggest that the lack of neurovirulence in RE6 is not due to a simple loss in the expression of a transcript or to a defect in a protein encoded by a gene at the site of recombination.
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Wagner E., in Roizman B. (ed.)Herpesviruses, vol. 3. Plenum Publishing Corp., New York, 1985, pp 45–104.
Wagner E., Costa R., Devi G., Draper K., Frink R., Hall L., Rice M. & Steinhart W., in Becker Y. (ed.),Herpesvirus mRNA, Developments in Molecular Virology, vol. 6. Martinus Nijhoff, The Hague, pp 79–99, 1985.
Swain M. & Galloway D., J Virol46, 1045–1050, 1983.
Draper K., Costa R., Lee G.T-Y., Spear P.G. & Wagner E.K., J Virol51, 578–585, 1984.
Swain M., Peet R. & Galloway D., J Virol53, 651–659, 1985.
Galloway D.A. & Swain M.A., J Virol49, 724–730, 1984.
Draper K.G., Devi-Rao G., Costa R.H., Blair E.D., Thompson R.L. & Wagner E.K., J Virol57, 1023–1038, 1986.
Thompson R.L., Wagner E.K. & Stevens J.G., Virology131, 180–192, 1983.
Costa R.H., Draper K.G., Devi-Rao G., Thompson R.L. & Wagner E.K., J Virol56, 19–30, 1985.
Maniatis T., Fritsch E.F. & Sambrook J.,Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY, 1982.
Sanger F., Nicklen S. & Coulson A.R., Proc Natl Acad Sci USA74, 5463, 1977.
Thompson R.L., Devi-Rao G.D., Stevens J.G. & Wagner E.K., J Virol55, 504–508, 1985.
Chandler V.L., Maler B.A., & Yamamoto K.R., Cell33, 489–499, 1983.
Pfahl M., McGinnis D., Hendricks M., Groner B. & Hynes N.E., Science222, 1341–1343, 1983.
Short J.M., Wynshaw-Boris A., Short H.P. & Hanson R.W., J Biol Chem261, 9721–9726, 1986.
Davison A.J. & Scott J.E., J Gen Virol67, 1759–1816, 1986.
Cortini R. and Wilkie N.M., J Gen Virol39, 259–280, 1978.
Preston V.G. and Fisher F.B., Virology138, 58–68, 1984.
Hall L.M., Draper K.G., Frink R.J., Costa R.H. & Wagner E.K., J Virol43, 594–607, 1982.
Schaffer P.A., Wagner E.K., Devi-Rao G.B. & Preston V.G., in Brian S.O. (ed.),Genetic Maps 1986. Cold Spring Harbor Laboratory Press, NY, (in press).
Debroy C., Pederson N. & Person S., Virology145, 36–48, 1985.
Draper K.G., Frink R.J., Devi G.B., Swain M., Galloway D. & Wagner E.K., J Virol52, 615–623, 1984.
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Thompson, R.L., Devi-Rao, G.V. & Wagner, E.K. DNA sequence and RNA transcription through a site of recombination in a non-neurovirulent herpes simplex virus intertypic recombinant. Virus Genes 1, 275–286 (1988). https://doi.org/10.1007/BF00572706
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DOI: https://doi.org/10.1007/BF00572706