Abstract
We have developed two strains of hooded rats with differing erythrocyte oxygen affinities by selection on red cell 2,3-diphosphoglycerate levels. Genetic studies have shown that these strains differ at one DPG-level-determining locus. This article reports the results of a study which involved measurement of plasma cholesterol levels in rats from the strains and the F2 progeny of strain intercrosses. Low-DPG strain rats, with high oxygen affinity, had significantly higher mean cholesterol levels than High-DPG rats. Animals from the extremes of the F2 distribution of DPG levels showed similar, significantly different mean cholesterol levels, indicating that the negative association between DPG and cholesterol levels in strain rats was not due to inadvertent fixation of unrelated genes during selection on DPG. The possibility is discussed that high oxygen affinity, brought about by low DPG levels, may be causative in increasing cholesterol levels.
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References
Benesch, R., and Benesch, R. E. (1967). The effect of organic phosphates from the human erythrocyte on the allosteric properties of hemoglobin. Biochem. Biophys. Res. Commun. 26162.
Brewer, G. J. (1972). Clinical implications of variation in erythrocyte oxygen affinity: A. Blood storage and B. Arteriosclerosis. In Astrup, P., and Rorth, M. (eds.), Oxygen Affinity and Red Cell Acid Base Status, Academic Press, New York, pp. 629–645.
Brewer, G. J., Oelshlegel, F. J., Jr., and Eaton, J. (1972). Biochemical, physiological and genetic factors in the regulation of mammalian erythrocyte metabolism and DPG levels. In Astrup, P., and Rorth, M. (eds.), Oxygen Affinity and Red Cell Acid Base Status, Academic Press, New York, pp. 539–551.
Chanutin, A., and Curnish, R. R. (1967). Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes. Arch. Biochem. 12196.
Keitt, A. (1971) Reduced nicotinamide adenine dinucleotide linked analysis of 2,3-DPG. J. Lab. Clin. Med. 77470.
Kelsey, H. (1939). Determination of cholesterol. J. Biol. Chem. 12715.
Noble, N., and Brewer, G. J. (1972). Studies of the metabolic basis of the ATP-DPG differences in genetically selected high and low ATP-DPG rat strains. In Brewer, G. J. (ed.), Hemoglobin and Red Cell Structure and Function, Plenum, New York, pp. 155–164.
Noble, N. A., and Brewer, G. J. (1977). Identification of a major locus contributing to erythrocyte 2,3-diphosphoglycerate variability in hooded (Long-Evans) rats. Genetics 85669.
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This work was supported by an NIH Training Grant (5-T01-GM-0071) and a Michigan Heart Association Grant.
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Noble, N.A., Brewer, G.J. & Oelshlegel, F.J. Association between cholesterol and 2,3-diphosphoglycerate in genetically selected hooded rat lines. Biochem Genet 16, 39–44 (1978). https://doi.org/10.1007/BF00484383
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DOI: https://doi.org/10.1007/BF00484383