Abstract
These experiments studied changes produced by a hypnotic dose of ethanol in the LS and SS lines of mice, which differ in ethanol sensitivity. In the first experiment, animals were injected either with ethanol or saline, and activity and seizure susceptibility measured 7–9 h later when blood levels of ethanol would have reached zero. Ethanol-treated mice of both genetic lines were less active in an open field test and more susceptible to clonic convulsions induced by flurothyl than saline-injected controls. There was no difference in the magnitude of these changes in the two lines. In the control condition SS (short-sleep) mice were more active than LS (long-sleep) mice, and more susceptible than LS mice to myoclonic but not to clonic seizures. The effect of the ethanol injection on body temperature was evaluated in separate groups of animals. LS mice showed a more pronounced hypothermia than LS mice when temperature was measured 2 h after injection. Six hours after injection, SS mice exhibited a small but statistically significant overshoot in temperature, after which they again became hypothermic with respect to controls; hyperthermia was not observed in LS animals.
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Church, A. C., Fuller, J. L., Dudek, B. C.: Salsolinol differentially affects mice selected for sensitivity to alcohol. Psychopharmacology 47, 49–52 (1976)
Erwin, V. G., Heston, W. D. W., McClearn, G. E., Deitrich, R. A.: Effect of hypnotics on mice genetically selected for sensitivity to ethanol. Pharmacol. Biochem. Behav. 4, 679–683 (1976)
Freund, G.: Hypothermia after acute ethanol and benzyl alcohol administration. Life Sci. 13, 345–349 (1973)
Greer, C. A., Alpern, H. P., Collins, A. C.: Increased CNS sensitivity to flurothyl as a measure of physical dependence in mice following morphine, phenobarbital, and ethanol treatment. Life Sci. 18, 1375–1382 (1976)
Greer, C. A., Alpern, H. P.: Mediation of myoclonic seizures by dopamine and clonic seizures by acetylcholine and GABA. Life Sci. 21, 385–392 (1977)
Goldstein, D. B.: Relationship of alcohol dose to intensity of withdrawal signs in mice. J. Pharmacol. Exp. Ther. 180, 203–215 (1972)
Goldstein, D. B., Kakihana, R.: Alcohol withdrawal reactions in mouse strains selectively bred for long or short sleep times. Life Sci. 17, 981–986 (1975)
Heston, W. D. W., Erwin, V. G., Anderson, S. M., Robbins, H.: A comparison of the effects of alcohol on mice selectively bred for differences in ethanol sleep time. Life Sci. 14, 365–370 (1974)
Isbell, H., Fraser, H. F., Wikler, A., Belleville, R. E., Eisenman, A. J.: An experimental study of the etiology of ‘rumfits’ and delirium tremens. Q. J. Stud. Alcohol 16, 1–33 (1955)
Kakihana, R., Moore, J.: Ethanol-induced hypo- and hyperthermia in inbred strains of mice and mice selectively bred for ethanol sensitivity. Life Sci. 23, 2331–2337 (1978)
Kalant, H., LeBlanc, A. E., Gibbins, R. J.: Tolerance to, and dependence on, some non-opiate psychotropic drugs. Pharmacol. Rev. 23, 135–191 (1971)
Majchrowicz, E.: Induction of physical dependence upon ethanol and the associated behavioral changes in rats. Psychopharmacology 43, 245–254 (1975)
McClearn, G. E., Kakihana, R.: Selective breeding for ethanol sensitivity in mice. Behav. Genet. 3, 409–410 (1973)
McQuarrie, D., Fingl, E.: Effects of single doses and chronic administration of ethanol on experimental seizures in mice. J. Pharmacol. Exp. Ther. 124, 264–271 (1958)
Pohorecky, L. A., Jaffe, L. S., Berkeley, H. A.: Effect of ethanol on serotonergic neurons in the rat brain. Res. Commun. Chem. Pathol. Pharmacol. 8, 1–11 (1974)
Pohorecky, L. A.: Withdrawal from ethanol: simple quantitative tests for its evaluation. Psychopharmacology 50, 125–129 (1976)
Ritzmann, R. F., Tabakoff, B.: Body temperature in mice: a quantitative measure of alcohol tolerance and physical dependence. J. Pharmacol. Exp. Ther. 199, 158–170 (1976)
Sanders, B.: Sensitivity to low doses of ethanol and pentobarbital in mice selected for sensitivity to hypnotic doses of ethanol. J. Comp. Physiol. Psychol. 90, 394–398 (1976)
Sanders, B., Sharpless, S. K.: Dissociation between the anticonvulsant action of alcohol and its depressant action in mice of different genotypes. Life Sci. 23, 2593–2600 (1978)
Schuck, S. L., Shulman, A.: A study of the central action of flurothyl and methoxyflurane. Aust. J. Exp. Biol. Med. Sci. 49, 501–512 (1971)
Siemens, A. J., Chan, A. W. K.: Differential effects of pentobarbital and ethanol in mice. Life Sci. 19, 581–589 (1976)
Truitt, E. B., Jr., Ebersberger, E. M., Ling, A. S. C.: Measurement of brain excitability by use of hexaflurodiethyl ether (Indoklon). J. Pharmacol. Exp. Ther. 129, 445–453 (1960)
Victor, M.: In: Clinical neurology, vol. II, A. B. Baker, ed., pp. 1084–1120. New York: Hoeber-Harper 1962
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Sanders, B. Withdrawal-like signs induced by a single administration of ethanol in mice that differ in ethanol sensitivity. Psychopharmacology 68, 109–113 (1980). https://doi.org/10.1007/BF00432126
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DOI: https://doi.org/10.1007/BF00432126