Abstract
Hybrid 70S ribosomes were produced by combining Anacystis nidulans and Escherichia coli 30S and 50S subunits. Both the A. nidulans 30S-E. coli 50S and E. coli 30S- A. nidulans 50S hybrids were functional in synthesizing protein when tested in a standard in vitro amino acid incorporating system. Both 70S hybrids were inhibited by streptomycin but the degree of inhibition was dependent upon the source of the 30S subunit. The ability to form functional 70S ribosomes from subunits of blue-green algae and bacteria is further evidence of the procaryotic nature of blue-greens and of the functional homology of the two protein synthesizing systems.
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Asato, Y., Ginoza, H. S.: Separation of small circular DNA molecules from the blue-green alga Anacystis nidulans. Nature New Biol. 244, 132–133 (1973)
Altenburg, L. C., Saunders, G. F.: Properties of hybrid ribosomes formed from the subunits of mesophilic and thermophilic bacteria. J. molec. Biol. 55, 487–502 (1971)
Bachmayer, H., Kreil, G.: Formation of N-formyl-methionylpuromycin by intact cells of four different bacteria and a bluegreen alga. Biochim. biophys. Acta (Amst.) 169, 95–102 (1968)
Coleman, G.: Effect of the magnesium concentration in the extracting medium on the polyribosome content and the cell-free amino acid incorporating ability of extracts of logarithmic phase cells of a Bacillus sp. Biochim. biophys. Acta (Amst.) 182, 180–192 (1969)
Craig, I. W., Carr, N. G.: Ribosomes from the blue-green alga Anabaena variabilis. Arch. Mikrobiol. 62, 167–177 (1968)
Grivell, L. A., Walg, H. L.: Subunit homology between Escherichia coli, mitochondrial and chloroplast ribosomes. Biochem. biophys. Res. Commun. 49, 1452–1458 (1972)
Heywood, S. M., Dowben, R. M., Rich, A.: A study of muscle polyribosomes and the coprecipitation of polyribosomes with myosin. Biochemistry 7, 3289–3296 (1968)
Kratz, W. A., Myers, J.: Nutrition and growth of several bluegreen algae. Amer. J. Bot. 42, 282–287 (1955)
Lee, S. G., Evans, W. R.: Hybrid ribosome formation from Escherichia coli and chloroplast ribosome subunits. Science 173, 241–242 (1971)
Loening, U. E.: Molecular weights of ribosomal RNA in relation to evolution. J. molec. Biol. 38, 355–365 (1968)
Makino, F., Tsuzuki, J.: Absence of histone in the blue-green alga Anabaena cylindrica. Nature (Lond.) 231, 446–447 (1971)
Martin, T. E., Wool, I. G.: Active hybrid 80S particles formed from subunits of rat, rabbit, and protozoan (Tetrahymena pyriformis) ribosomes. J. molec. Biol. 43, 151–161 (1969)
Nirenberg, M. W., Matthaei, J. H.: The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Proc. nat. Acad. Sci. (Wash.) 47, 1588–1602 (1961)
Noll, H., Staehelin, T., Wettstein, F. O.: Ribosomal aggregates engaged in protein synthesis: ergosome breakdown and messenger ribonucleic acid transport. Nature (Lond.) 198, 632–638 (1963)
Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin resistant locus in E. coli. Nature (Lond.) 222, 333–336 (1969)
Ris, H., Singh, R. N.: Electron microscope studies on blue-green algae. J. biophys. biochem. Cytol. 9, 63–80 (1961)
Spirin, A. S.: On the equilibrium of the association-dissociation reaction of ribosomal subparticles and on the existence of the so-called ’60S intermediate’ (‘swollen 70S’) during centrifugation of the equilibrium mixture. FEBS Letters 14, 349–353 (1971)
Stanier, R. Y., Kunisawa, R., Mandel, M., Cohen-Bazire, G.: Purification and properties of unicellular blue-green algae (Order Chroococcales). Bact. Rev. 35, 171–205 (1971)
Stanier, R. Y., van Niel, C. B.: The concept of a bacterium. Arch. Mikrobiol. 42, 17–35 (1962)
Stanley, W. M., Salas, M., Wahba, A. J., Ochoa, S.: Translation of the genetic message: Factors involved in the initiation of protein synthesis. Proc. nat. Acad. Sci. (Wash.) 56, 290–295 (1966)
Takeda, M., Lipmann, F.: Comparison of amino acid polymerization in B. subtilis and E. coli cell free systems; hybridization of their ribosomes. Proc. nat. Acad. Sci. (Wash.) 56, 1875–1882 (1966)
Taylor, M. M., Storck, R.: Uniqueness of bacterial ribosomes. Proc. nat. Acad. Sci. (Wash.) 52, 958–965 (1964)
Wolk, C. P.: Physiology and cytological chemistry of blue-green algae. Bact. Rev. 37, 32–101 (1973)
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Gray, J.E., Herson, D.S. Functional 70S hybrid ribosomes from blue-green algae and bacteria. Arch. Microbiol. 109, 95–99 (1976). https://doi.org/10.1007/BF00425118
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DOI: https://doi.org/10.1007/BF00425118