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Functional 70S hybrid ribosomes from blue-green algae and bacteria

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Abstract

Hybrid 70S ribosomes were produced by combining Anacystis nidulans and Escherichia coli 30S and 50S subunits. Both the A. nidulans 30S-E. coli 50S and E. coli 30S- A. nidulans 50S hybrids were functional in synthesizing protein when tested in a standard in vitro amino acid incorporating system. Both 70S hybrids were inhibited by streptomycin but the degree of inhibition was dependent upon the source of the 30S subunit. The ability to form functional 70S ribosomes from subunits of blue-green algae and bacteria is further evidence of the procaryotic nature of blue-greens and of the functional homology of the two protein synthesizing systems.

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References

  • Asato, Y., Ginoza, H. S.: Separation of small circular DNA molecules from the blue-green alga Anacystis nidulans. Nature New Biol. 244, 132–133 (1973)

    Google Scholar 

  • Altenburg, L. C., Saunders, G. F.: Properties of hybrid ribosomes formed from the subunits of mesophilic and thermophilic bacteria. J. molec. Biol. 55, 487–502 (1971)

    Google Scholar 

  • Bachmayer, H., Kreil, G.: Formation of N-formyl-methionylpuromycin by intact cells of four different bacteria and a bluegreen alga. Biochim. biophys. Acta (Amst.) 169, 95–102 (1968)

    Google Scholar 

  • Coleman, G.: Effect of the magnesium concentration in the extracting medium on the polyribosome content and the cell-free amino acid incorporating ability of extracts of logarithmic phase cells of a Bacillus sp. Biochim. biophys. Acta (Amst.) 182, 180–192 (1969)

    Google Scholar 

  • Craig, I. W., Carr, N. G.: Ribosomes from the blue-green alga Anabaena variabilis. Arch. Mikrobiol. 62, 167–177 (1968)

    Google Scholar 

  • Grivell, L. A., Walg, H. L.: Subunit homology between Escherichia coli, mitochondrial and chloroplast ribosomes. Biochem. biophys. Res. Commun. 49, 1452–1458 (1972)

    Google Scholar 

  • Heywood, S. M., Dowben, R. M., Rich, A.: A study of muscle polyribosomes and the coprecipitation of polyribosomes with myosin. Biochemistry 7, 3289–3296 (1968)

    Google Scholar 

  • Kratz, W. A., Myers, J.: Nutrition and growth of several bluegreen algae. Amer. J. Bot. 42, 282–287 (1955)

    Google Scholar 

  • Lee, S. G., Evans, W. R.: Hybrid ribosome formation from Escherichia coli and chloroplast ribosome subunits. Science 173, 241–242 (1971)

    Google Scholar 

  • Loening, U. E.: Molecular weights of ribosomal RNA in relation to evolution. J. molec. Biol. 38, 355–365 (1968)

    Google Scholar 

  • Makino, F., Tsuzuki, J.: Absence of histone in the blue-green alga Anabaena cylindrica. Nature (Lond.) 231, 446–447 (1971)

    Google Scholar 

  • Martin, T. E., Wool, I. G.: Active hybrid 80S particles formed from subunits of rat, rabbit, and protozoan (Tetrahymena pyriformis) ribosomes. J. molec. Biol. 43, 151–161 (1969)

    Google Scholar 

  • Nirenberg, M. W., Matthaei, J. H.: The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Proc. nat. Acad. Sci. (Wash.) 47, 1588–1602 (1961)

    Google Scholar 

  • Noll, H., Staehelin, T., Wettstein, F. O.: Ribosomal aggregates engaged in protein synthesis: ergosome breakdown and messenger ribonucleic acid transport. Nature (Lond.) 198, 632–638 (1963)

    Google Scholar 

  • Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin resistant locus in E. coli. Nature (Lond.) 222, 333–336 (1969)

    Google Scholar 

  • Ris, H., Singh, R. N.: Electron microscope studies on blue-green algae. J. biophys. biochem. Cytol. 9, 63–80 (1961)

    Google Scholar 

  • Spirin, A. S.: On the equilibrium of the association-dissociation reaction of ribosomal subparticles and on the existence of the so-called ’60S intermediate’ (‘swollen 70S’) during centrifugation of the equilibrium mixture. FEBS Letters 14, 349–353 (1971)

    Google Scholar 

  • Stanier, R. Y., Kunisawa, R., Mandel, M., Cohen-Bazire, G.: Purification and properties of unicellular blue-green algae (Order Chroococcales). Bact. Rev. 35, 171–205 (1971)

    Google Scholar 

  • Stanier, R. Y., van Niel, C. B.: The concept of a bacterium. Arch. Mikrobiol. 42, 17–35 (1962)

    Google Scholar 

  • Stanley, W. M., Salas, M., Wahba, A. J., Ochoa, S.: Translation of the genetic message: Factors involved in the initiation of protein synthesis. Proc. nat. Acad. Sci. (Wash.) 56, 290–295 (1966)

    Google Scholar 

  • Takeda, M., Lipmann, F.: Comparison of amino acid polymerization in B. subtilis and E. coli cell free systems; hybridization of their ribosomes. Proc. nat. Acad. Sci. (Wash.) 56, 1875–1882 (1966)

    Google Scholar 

  • Taylor, M. M., Storck, R.: Uniqueness of bacterial ribosomes. Proc. nat. Acad. Sci. (Wash.) 52, 958–965 (1964)

    Google Scholar 

  • Wolk, C. P.: Physiology and cytological chemistry of blue-green algae. Bact. Rev. 37, 32–101 (1973)

    Google Scholar 

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Gray, J.E., Herson, D.S. Functional 70S hybrid ribosomes from blue-green algae and bacteria. Arch. Microbiol. 109, 95–99 (1976). https://doi.org/10.1007/BF00425118

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  • DOI: https://doi.org/10.1007/BF00425118

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