Abstract
Growth of Methanobacterium thermoautotrophicum on H2 and CO2 as sole energy and carbon sources was found to be dependent on Ni, Co, and Mo. At low concentrations of Ni (<100 nM), Co (<10 nM) and Mo (<10 nM) the amount of cells formed was roughly proportional to the amount of transition metal added to the medium; for the formation of 1 g cells (dry weight) approximately 150 nmol NiCl2, 20 nmol CoCl2 and 20 nmol Na2MoO4 were required. A dependence of growth on Cu, Mn, Zn, Ca, Al, and B could not be demonstrated. Conditions are described under which the bacterium grew exponentially with a doubling time of 1.8 h up to a cell density of 2 g cells (dry weight)/1.
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Schönheit, P., Moll, J. & Thauer, R.K. Nickel, cobalt, and molybdenum requirement for growth of Methanobacterium thermoautotrophicum . Arch. Microbiol. 123, 105–107 (1979). https://doi.org/10.1007/BF00403508
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DOI: https://doi.org/10.1007/BF00403508