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Photoacoustic and fluorescence measurements of the chilling response and their relationship to carbon dioxide uptake in tomato plants

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Abstract

The response of tomato plants to various chilling treatments was studied using two approaches for the measurement of photosynthetic activity. One involved the use of a portable fluorometer for the measurement of in-vivo chlorophyll fluorescence, while the other employed a newly introduced photoacoustic system which allowed changes in oxygen evolution to be followed in a leaf disc. A strong correlation was found between results obtained by each system and those obtained by a conventional open gas-exchange system for the determination of CO2 uptake. Both systems of measurements could readily distinguish between the effects of chilling in the dark (at 3° C for 18 h) and chilling at high photon flux density (2000 μmol m-2 s-1 for 5h at 5° C). Chilling in the dark had practically no effect on the quantum yield of oxygen evolution, chlorophyll fluorescence or CO2 uptake, while chilling at excessively high photon flux density resulted in a sharp reduction (50–70%) in the quantum yields obtained. The results support the view that photosystem II cannot be the primary site of damage by chilling in the dark, although it is significantly affected by chilling at high light intensity.

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Abbreviations

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

PA:

photoacoustic

PFD:

photon flux density

PSII:

photosystem II

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Authors and Affiliations

  1. Faculty of Agriculture, Department of Field and Vegetable Crops, Hebrew University of Jerusalem, P.O. Box 12, 76-100, Rehovot, Israel

    Dan Yakir, Jehoshua Rudich & Ben-Ami Bravdo

Authors
  1. Dan Yakir
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  2. Jehoshua Rudich
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  3. Ben-Ami Bravdo
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Yakir, D., Rudich, J. & Bravdo, BA. Photoacoustic and fluorescence measurements of the chilling response and their relationship to carbon dioxide uptake in tomato plants. Planta 164, 345–353 (1985). https://doi.org/10.1007/BF00402945

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  • DOI: https://doi.org/10.1007/BF00402945

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