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Effect of light quality on chloroplast-membrane organization and function in pea

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Abstract

The effect of light quality during plant growth of chloroplast membrane organization and function in peas (Pisum sativum L. cv. Alaska) was investigated. In plants grown under photosystem (PS) I-enriched (far-red enriched) illumination both the PSII/PSI stoichiometry and the electrontransport capacity ratios were high, about 1.9. In plants grown under PSII-enriched (far-red depleted) illumination both the PSII/PSI stoichiometry and the electron-transport capacity ratios were significantly lower, about 1.3. In agreement, steady-state electron-transport measurements under synchronous illumination of PSII and PSI demonstrated an excess of PSII in plants grown under far-red-enriched light. Sodium dodecylsulfate polyacrylamide gel electrophoretic analysis of chlorophyll-containing complexes showed greater relative amounts of the PSII reaction center chlorophyll-protein complex in plants grown under farred-enriched light. Additional changes were observed in the ratio of light-harvesting chlorophyll a/b protein to PSII reaction center chlorophyll-protein under the two different light-quality regimes. The results demonstrate the dynamic nature of chloroplast structure and support the notion that light quality is an important factor in the regulation of chloroplast membrane organization and-function.

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Abbreviations

Chl:

chlorophyll

CPa:

PSII reaction center chlorophyll protein complex

CPI:

PSI chlorophyll protein complex

FR-D:

light depleted in far-red sensitizing primarily PSII

FR-E:

light enriched in far-red sensitizing primarily PSI

LHCP:

PSII light-harvesting chlorophyll a/b protein complex

P 700 :

primary electron donor of PSI

PSI, PSII:

photosystems I and II, respectively

Q :

primary electron acceptor of PSII

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Authors and Affiliations

  1. Division of Molecular Plant Biology, University of California, 313 Hilgard Hall, 94720, Berkeley, CA, USA

    R. E. Glick, S. W. McCauley & A. Melis

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  1. R. E. Glick
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  2. S. W. McCauley
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  3. A. Melis
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Glick, R.E., McCauley, S.W. & Melis, A. Effect of light quality on chloroplast-membrane organization and function in pea. Planta 164, 487–494 (1985). https://doi.org/10.1007/BF00395964

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  • DOI: https://doi.org/10.1007/BF00395964

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