Skip to main content
SpringerLink
Log in

Fast photoacoustic transients from dark-adapted intact leaves: oxygen evolution and uptake pulses during photosynthetic induction — a phenomenology record

  • Published:
Planta Aims and scope Submit manuscript

Abstract

Using a photoacoustic technique it has been possible to observe fast oxygen evolution and uptake transients at a high time resolution (approx. 0.2 s), when a dark-adapted leaf is reilluminated. There is initially a rapid pulse of oxygen evolution, correlated with the initial fluorescence rise (total duration under the experimental conditions used about 1–2 s), corresponding presumably to the photoreduction of the plastoquinone pool. This phenomenon may be utilized to calibrate the oxygen-evolution photoacoustic signal. The first pulse is followed by a series of slower bursts of oxygen uptake and evolution, reflecting various pools which are expressed following sequential activation of various parts of the photosynthetic apparatus, until achievement of a steady state.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

RuBP:

ribulose-1,5-bisphosphate

PSI, PSII:

photosystems I, II

Fo, Fm, F(t):

initial, maximum and instantaneous chlorophyll fluorescence emission

References

  • Anderson, L.E. (1979) Interaction between photochemistry and activity of enzymes. In: Encyclopedia of plant physiology, N.S., vol. 2: Photosynthesis, pp. 271–281, Gibbs, M., Latzko, E., eds. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Behrens, P.W., Marsho, T.V., Radmer, R.J. (1982) Photosynthetic O2 exchange kinetics in isolated soybean cells. Plant Physiol. 70, 179–185

    Google Scholar 

  • Blinks, L.R., Skow, R.K. (1938) The time course of photosynthesis as shown by a rapid electrode method for oxygen. Proc. Natl. Acad. Sci. USA 24, 420–427

    Google Scholar 

  • Buchanan, B.B. (1980) Role of light in the regulation of chloroplast enzymes. Annu. Rev. Plant Physiol. 31, 341–374

    Google Scholar 

  • Bults, G., Horwitz, B.A., Malkin, S., Cahen, D. (1982) Photoacoustic measurements of photosynthetic activities in whole leaves — photochemistry and gas exchange. Biochim. Biophys. Acta 679, 452–465

    Google Scholar 

  • Canaani, O., Malkin, S. (1984) Physiological adaptation to low light intensity in intact leaves resulting in an extreme imbalanced light distribution between the two photosystems of photosynthesis. Biochim. Biophys. Acta 766, 525–532

    Google Scholar 

  • Carillo, N., Lucero, H.A., Vallejos, R.H. (1981) Light modulation of chloroplast-bound Ferredoxin-NADP+ reductase. J. Biol. Chem. 256, 1058–1059

    Google Scholar 

  • Canvin, D.T., Berry, J.A., Badger, M.R., Foch, H., Osmond, C.B. (1980) Oxygen exchange in leaves in the light. Plant Physiol. 66, 302–307

    Google Scholar 

  • Ehleringer, J., Björkman, O. (1977) Quantum yields for CO2 uptake in C3 and C4 plants. Plant Physiol. 59, 86–90

    Google Scholar 

  • Forbush, B., Kok, B., McGloin, M. (1971) Cooperation of charges in photosynthetic O2 evolution. II. Damping of flash yield oscillation, deactivation. Photochem. Photobiol. 14, 307–321

    Google Scholar 

  • Furbank, R.T., Badger, M.R., Osmond, C.B. (1982) Photosynthetic oxygen exchange in isolated cells and chloroplasts of C3 plants. Plant Physiol. 70, 827–931

    Google Scholar 

  • Furbank, R.T., Badger, M.R., Osmond, C.B. (1983) Photoreduction of oxygen in mesophyll chloroplasts of C4 plants — a model system for studying an in-vivo Mehler reaction. Plant Physiol. 73, 1038–1041

    Google Scholar 

  • Heldt, H.W., Laing, W., Lorimer, G.H., Stitt, M., Wirz, W. (1981) On the regulation of CO2 fixation by light. In: Photosynthesis, vol. VI: Regulation of carbon metabolism, pp. 213–226, Akoyunoglou, G., ed. Balaban Int. Serv., Philadelphia

    Google Scholar 

  • Hoch, G., Owens, O.H., Kok, B. (1963) Photosynthesis and respiration. Arch. Biochem. Biophys. 101, 171–180

    Google Scholar 

  • Horton, P. (1983) Effects of changes in the capacity for photosynthetic electron transfer and photophosphorylation on the kinetics of fluorescence induction in isolated chloroplasts. Biochim. Biophys. Acta 724, 404–410

    Google Scholar 

  • Inoue, Y., Watanabe, A., Shibata, K. (1979) Transient variation of photoacoustic signal from leaves accompanying photosynthesis. FEBS Lett. 101, 321–323

    Google Scholar 

  • Joliot P. (1960) Contribution a l'étude des phénomènes d'induction de la photosynthèse. Thèse de Doctorat, Université de Paris

  • Joliot, P., Joliot, A. (1968) A polarographic method for the detection of oxygen production and reduction of Hill reagent by isolated chloroplasts. Biochim. Biophys. Acta 153, 625–634

    Google Scholar 

  • Kelly, G.J., Latzko, E., Gibbs, M. (1976) Regulatory aspects of photosynthetic carbon metabolism. Annu. Rev. Plant Physiol. 27, 181–205

    Google Scholar 

  • Kok, B., Cheniae, G.M. (1966) Kinetics and intermediates of the oxygen evolution step in photosynthesis. Curr. Top. Bioenerg. 1, pp 2–47

    Google Scholar 

  • Kok, B., Forbush, B., McGloin, M. (1970) Cooperation of charges in photosynthetic O2 evolution. I. A linear fourstep mechanism. Photochem. Photobiol. 11, 457–475

    Google Scholar 

  • Lavorel, J., Etienne, A.L. (1977) In vivo chlorophyll fluorescence. In: Topics in photosynthesis, vol. 2: Primary processes of photosynthesis, pp. 203–268, Barber, J., ed. Elsevier/North Holland Biomedical Press, Amsterdam

    Google Scholar 

  • Leegood, R.C., Walker, D.A. (1980) Autocatalysis and light activation of enzymes in relation to photosynthetic induction in wheat chloroplasts. Arch. Biochem. Biophys. 200, 575–582

    Google Scholar 

  • Leegood, R.C., Walker, D.A. (1981) Photosynthetic induction in wheat protoplasts and chloroplasts. Autocatalysis and light activation of enzymes. Plant Cell Environ. 4, 59–66

    Google Scholar 

  • Malkin, S., Kok, B. (1966) Fluorescence induction studies in isolated chloroplasts I. Number of components involved in the reaction and quantum yield. Biochim. Biophys. Acta 126, 413–436

    Google Scholar 

  • Osterhout, W.J.V., Haas, A.R.C. (1918) On the dynamics of photosynthesis. J. Gen. Physiol. 1, 1–16

    Google Scholar 

  • Poulet, P., Cahen, D., Malkin, S. (1983) Photoacoustic detection of photosynthetic oxygen evolution from leaves —Quantitative analysis by phase and amplitude measurements. Biochim. Biophys. Acta 724, 433–446

    Google Scholar 

  • Prinsley, R.T., Leegood, R.C. (1986) Factors affecting photosynthetic induction in spinach leaves. Biochim. Biophys. Acta 849, 244–253

    Google Scholar 

  • Prinsley, R.T., Heath, R.L., Walker, D.A. (1984) Induction of photosynthetic oxygen evolution in spinach leaves. In: Advances in photosynthesis research, vol. III, pp. 653–656, Sybesma, C. (ed.) Martinus Nijhoff/Dr. Junk pub., The Hague, Boston Lancaster

    Google Scholar 

  • Rabinowich, E.I. (1956) Photosynthesis and related processes, vol. II, pt. 2, pp. 1313, Interscience, New York

    Google Scholar 

  • Vu, J., Cu, V., Allen, L.H., Bowes, G. (1984) Dark/light modulation of ribulosebisphosphate carboxylase activity in plants from different photosynthetic categories. Plant Physiol. 76, 843–845

    Google Scholar 

  • Walker, D.A. (1976) CO2 fixation by intact chloroplasts: photosynthetic induction and its relation to transport phenomena and control mechanisms. In: Topics in photosynthesis, vol. 1: The intact chloroplast (chpt. 7), pp. 235–278, Barber, J., ed. Elsevier, Amsterdam

    Google Scholar 

  • Walker, D.A. (1980) Preparation of higher plant chloroplasts. Methods Enzymol. 69, 94–104

    Google Scholar 

  • Walker, D.A., Sivak, M.N., Prinsley, R.T., Cheesbrough, J.K. (1983) Simultaneous measurement of oscillations in oxygen evolution and chlorophyll-a fluorescence in leaf pieces. Plant Physiol. 73, 542–549

    Google Scholar 

Download references

Author information

Author notes

  1. S. Malkin

    Present address: Biochemistry Department, Weizmann Institute of Science, 76100, Rehovot, Israel

Authors and Affiliations

  1. Research Institute for Photosynthesis, University of Sheffield, S10 2TN, Sheffield, UK

    S. Malkin

Authors
  1. S. Malkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Malkin, S. Fast photoacoustic transients from dark-adapted intact leaves: oxygen evolution and uptake pulses during photosynthetic induction — a phenomenology record. Planta 171, 65–72 (1987). https://doi.org/10.1007/BF00395068

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00395068

Key words

Search

Navigation