Abstract
The phosphorylation of thylakoid proteins, which comprise apoproteins of the light-harvesting chlorophyll a/b-protein complex (LHCP), was investigated in vivo and in vitro during the development of Scenedesmus obliquus in synchronous cultures. The in-vitro and in-vivo protein phosphorylation exhibited a maximum activity in cells with maximum photosynthetic capacity (8th hour) and miximum activity in cells with minimum photosynthetic capacity (16th hour). The major phosphorylated polypeptides in vivo were the 24/25-kDa and 28–30-kDa apoprotein of the LHCP, a protein of about 32 kDa, and some smaller polypeptides within the range 10 to 20 kDa. In vitro, the main phosphoproteins were the 28–30-kDa apoprotein and the protein characterized by an apparent molecular weight of 32 kDa. Pulse-chase experiments in vivo established that the latter had the fastest radioactivity turnover of the thylakoidal phosphoproteins.
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Abbreviations
- DCMU:
-
3-(3′,4′-dichlorophenyl)-1,1-dimethylurea
- LHCP:
-
light-harvesting chlorophyll a/b-protein complex
- PSII:
-
photosystem II
References
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Dedicated to Prof. Erwin Bünning on the occasion of his 80th birthday
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Hei, W.G., Senger, H. Thylakoid-protein phosphorylation during the life cycle of Scenedesmus obliquus in synchronous culture. Planta 167, 233–239 (1986). https://doi.org/10.1007/BF00391420
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DOI: https://doi.org/10.1007/BF00391420