Summary
Two protein factors, isolated from S-100 of maize shoots, were found to be required not only for a “Mg2+ shift” for poly U-dependent polymerization of phenylalanine, but also for polyphenylalanine synthesis. They seem to be crude preparations of EF-1 and EF-2. The poly U-directed amino acid incorporation by washed ribosomes was completely dependent upon these factors.
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Abbreviations
- EF-1:
-
Elongation factor 1
- EF-2:
-
Elongation factor 2
References
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Sim, WS., Klämbt, D. Isolation of two protein factors from maize involved in poly U-dependent polyphenylalanine synthesis by maize ribosomes. Planta 131, 47–50 (1976). https://doi.org/10.1007/BF00387344
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DOI: https://doi.org/10.1007/BF00387344