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Shear activated channels in cell-attached patches of cultured bovine aortic endothelial cells

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  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Abstract

We investigated the response of inward rectifier K+ (IRK) currents in bovine aortic endothelial cells (BAECs) to shear stress. Shear evoked reversible hyperpolarization in current clamped BAECs. Voltage clamped BAECs exhibited large inward and small outward whole cell K+ currents blocked by cesium and increased in amplitude by exposure to shear stress. The open state probability of IRK channels in cell-attached membrane patches was increased within minutes of exposure to shear stress. IRK channels in inside-out patches were activated by increases in [Ca2+]i from 10−7 to 10−6 mM. We demonstrate that shear stress induces hyperpolarization and gating of single channel and whole cell IRK currents in BAECs.

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Authors and Affiliations

  1. Departments of Physiology and Medicine MCW Clinic at Froedtert, Medical College of Wisconsin, 9200 West Wisconsin Avenue, 53226, Milwaukee, WI, USA

    Elizabeth R. Jacobs, Debebe Gebremedhin, Eric K. Birks, Peter F. Davies & David R. Harder

  2. Faculté des Sciences et Techniques Laboratoires D'electrophysiologie Cellulaires, Université de Tours, F-37200, Paro de Grandmont, Tours, France

    Christine Cheliakine

  3. Department of Pathology, University of Chicago, M.C. 6079, 5841 South Maryland Avenue, 60637-1470, Chicago, IL, USA

    Peter F. Davies

Authors
  1. Elizabeth R. Jacobs
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  2. Christine Cheliakine
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  3. Debebe Gebremedhin
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  4. Eric K. Birks
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  5. Peter F. Davies
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  6. David R. Harder
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Additional information

Supported by NIH grant numbers K08-1924, HL49294 (ERJ), HL 29587-09 (DH), R37 HL 33833-07 (DH), and HL15062 (PFD).

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Jacobs, E.R., Cheliakine, C., Gebremedhin, D. et al. Shear activated channels in cell-attached patches of cultured bovine aortic endothelial cells. Pflügers Arch. 431, 129–131 (1995). https://doi.org/10.1007/BF00374386

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  • DOI: https://doi.org/10.1007/BF00374386

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