Summary
Southern hybridization analysis using homologous maize probes indicated that orf25 and coxIII are closely linked in the mitochondrial genome of rice (Oryza sativa) cultivar IR36. The two coding regions were found on the same 5.1 kb BamHI fragment, and this fragment was cloned, mapped and partially sequenced. Using probes for each gene derived from the rice clone, a 2.4 kb dicistronic mRNA transcript was found containing both orf25 and coxIII coding regions. Multiple 5′ ends were identified by primer extension analysis and a double stem/loop structure was mapped to the 3′ end. The orf25 coding region shares >85% identity with orf25 sequences from maize, tobacco and wheat, suggesting that orf25 may code for a conserved protein product.
Similar content being viewed by others
References
Adams CC, Stern DB (1990) Nucleic Acids Res 18:6003–6010
Aiba H, Adhya S, de Crombrugghe B (1981) J Biol Chem 256:11905–11910
Barkan A (1988) EMBO J 7:2637–2644
Bland MM, Levings CS, Matzinger DF (1986) Mol Gen Genet 204:8–16
Bonen L, Bird S, Belanger L (1990) Plant Mol Biol 15:793–795
Dawson AJ, Jones VP, Leaver CJ (1984) EMBO J 3:2107–2113
Dewey RE, Levings CS III, Timothy DH (1986) Cell 44:439–449
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Gruissem W, Barkan A, Deng XW, Stern DB (1988) Trends Genet 4:258–263
Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) Mol Gen Genet 215:118–127
Hanic-Joyce PJ, Gray MW (1991) Mol Cell Biol 11:2035–2039
Hiesel R, Schobel W, Schuster W, Brennicke A (1987) EMBO J 6:29–34
Kaleikau EK, André CP, Walbot V (1990) Nucleic Acids Res 18:371
Kemble RJ, Bedbrook JR (1980) Nature 284:565–566
Luehrsen KR, Walbot V (1991) Mol Gen Genet 225:81–93
Maloney AP, Walbot V (1990) J Mol Biol 213:633–649
Maloney AP, Traynor PL, Levings CS III, Walbot V (1989) Curr Genet 15:207–212
McCarty DM, Heman GL, Hauswirth WW (1988) Nucleic Acids Res 16:9873
Mulligan RM, Maloney AP, Walbot V (1988a) Mol Gen Genet 211:373–380
Mulligan RM, Lau GT, Walbot V (1988b) Proc Natl Acad Sci USA 85:7998–8002
Mulligan RM, Dement E, André CP, Walbot V (1988c) Rice Genet Newsl 5:151–152
Mulligan RM, Leon PL, Walbot V (1991) Mol Cell Biol 11:533–543
Quagliariello C, Saiardi A, Gallerani R (1990) Curr Genet 18:355–363
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) J Mol Biol 143:161–178
Schwartz RM, Dayhoff MO (1978) Science 199:395–403
Schuster W, Hiesel R, Issac PG, Leaver CJ, Brennicke A (1986) Nucleic Acids Res 14:5943–5954
Stamper SE, Dewey RE, Bland MM, Levings CS III (1987) Curr Genet 12:457–463
Stern DB, Gruissem W (1987) Cell 51:1145–1157
Wahleithner JA, Wolstenholme DR (1988) Nucleic Acids Res 16:6897–6913
Wissinger B, Hissel R, Schuster W, Brennicke A (1988) Mol Gen Genet 212:56–57
Author information
Authors and Affiliations
Additional information
Communicated by D.R. Wolstenholme
Rights and permissions
About this article
Cite this article
Liu, A.W., Narayanan, K.K., André, C.P. et al. Co-transcription of orf25 and coxIII in rice mitochondria. Curr Genet 21, 507–513 (1992). https://doi.org/10.1007/BF00351661
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00351661