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Organization of a 117-kb circular mitochondrial chromosome in IR36 rice

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Abstract

The organization of the mitochondrial genome in the IR36 indica variety of rice was analyzed using constant-field and CHEF gel electrophoresis. The minimum complexity of the genome is estimated to be 300 kb. There was no evidence for a master circular molecule of this size. The genome exists in vivo in multiple subgenomic circular molecules or mitochondrial chromosomes. Using several rare-cutting restriction enzymes a restriction map was constructed for a 117-kb circular chromosome. Six genetic loci have been placed on this chromosome; cox1 and atp1 have been assigned precise positions while four additional genes (rrn26, cob2, atp6 and rrn18) were mapped to restriction fragments.

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Communicated by B. B. Sears

Present address: Center for Biotechnology, SPIC Science Foundation, 110 Mount Road, Madras 600032, India

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Narayanan, K.K., André, C.P., Yang, J. et al. Organization of a 117-kb circular mitochondrial chromosome in IR36 rice. Curr Genet 23, 248–254 (1993). https://doi.org/10.1007/BF00351503

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  • DOI: https://doi.org/10.1007/BF00351503

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