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Monoclonal antibodies against two separate alloantigenic sites of HLA-1340

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Abstract

Two monoclonal antibodies (MB40.2 and MB40.3) which are highly specific for HLA-B40 and HLA-B7 were made. They appear to be directed against two separate alloantigenic sites of these HLA molecules. Semiquantitative analysis of the kinetics of antibody binding show that MB40.2 recognizes a site which shows a degree of cross-reactivity with B7 and is specific to some B40 molecules. This antibody also distinguishes between different molecules typed as B40. In contrast, MB40.3 recognizes an antigenic determinant which is less variable between B7 and B40 and more closely approximates a public antigen or common antigenic site. This study suggests that the introduction of monoclonal antibodies into MHC serology not only permits but demands a quantitative analysis of these complex systems of homologous but highly polymorphic molecules.

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Abbreviations

MHC:

major histocompatibility complex

IgG:

immunoglobulin G

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

PBS:

phosphate-buffered saline

BSA:

bovine serum albumin

FCS:

fetal calf serum

RAM:

rabbit anti-mouse IgG F(ab′)2 fragments

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Authors and Affiliations

  1. Department of Structural Biology, Stanford University School of Medicine, 94305, Stanford, California

    Peter Parham

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  1. Peter Parham
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Additional information

For convenience the terms HLA antigen and H-2 antigen will refer only to the β2-microglobulin-associated, class I molecules coded for by the major histocompatibility complex of man and the mouse, respectively.

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Parham, P. Monoclonal antibodies against two separate alloantigenic sites of HLA-1340. Immunogenetics 13, 509–527 (1981). https://doi.org/10.1007/BF00343719

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  • DOI: https://doi.org/10.1007/BF00343719

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