Summary
Using the ABC immunohistochemical method, we investigated the distribution of calbindinlike immunoreactive structures in the optic tectum of normal fish, Tinca tinca, and from normal and unilaterally eye-enucleated fish, Cyprinus carpio. In nonoperated individuals of both species the optic tectum contained numerous immunoreactive neurons with strongly positive somata located in the stratum periventriculare and a thick immunolabeled dendritic shaft ascending radially toward the stratum fibrosum et griseum superficiale. The retinorecipient layers contained many fibrous immunoreactive structures. Some varicose fibers, isolated or in small bundles, were localized to the stratum album centrale, especially in the dorsal tectal half. Unilateral eye removal produced the disappearance of the immunoreactive fibrous structures located in the retinorecipient layers of the tectum contralateral to the enucleation. The present work shows that calbindinlike immunoreactive substances are localized in specific neural circuits of the fish optic tectum and suggests that the calbindin-like immunoreactive fibers in the retinorecipient strata are of retinal origin.
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References
Baimbridge KG, Miller JJ, Parkes CO (1982) Calcium-binding protein distribution in the rat brain. Brain Res 239:519–525
Braun K, Scheich H, Zuschratter W, Heizmann CW, Matute C, Streit P (1988) Postnatal development of parvalbumin-, calbindin-and adult GABA-immunoreactivity in two visual nuclei of zebra finches. Brain Res 475:205–217
DiFiglia M, Christakos S, Aronin N (1989) Ultrastructural localization of immunoreactive calbindin-D28k in the rat and monkey basal ganglia, including subcellular distribution with colloidal gold labeling. J Comp Neurol 279:653–665
Ekström P (1987) Distribution of choline acetyltransferase-immunoreactive neurons in the brain of a cyprinid teleost (Phoxinus phoxinus L.). J Comp Neurol 256:494–515
Enderlin S, Norman AW, Celio MR (1987) Ontogeny of the calcium binding protein calbindin D-28k in the rat nervous system. J Anat Embryol 177:15–28
Hamano K, Kiyama H, Emson PC, Manabe R, Nakauchi M, Tohyama M (1990) Localization of two calcium binding proteins, calbindin (28kD) and parvalbumin (12kD), in the vertebrate retina. J Comp Neurol 302:417–424
Hunziker W, Schrickel S (1988) Rat brain calbindin D28: six domain structure and extensive amino acid homology with chicken calbindin D28. Mol Endocrinol 2:465–473
Ichimiya Y, Emson PC, Mountjoy CQ, Lawson DEM, Iizuka R (1989) Calbindin-immunoreactive cholinergic neurons in the nucleus basalis of Meynert in Alzheimer-type dementia. Brain Res 499:402–406
Jones EG, Hendry SHC (1989) Differential calcium binding protein immunoreactivity distinguishes classes of relay neurons in monkey thalamic nuclei. Eur J Neurosci 1:222–246
Kretsinger RH, Mann JE, Simmonds JG (1982) Model of facilitated diffusion of calcium by the intestinal calcium-binding protein. In: Norman AW, Schaefer K, Grigoleit HG, Herrath DV (eds) Vitamin D, chemical, biochemical and clinical endocrinology of calcium metabolism. Walter de Gruyter, Berlin New York, pp 233–248
Marotte LR (1981) Density of optic terminals in half tecta of goldfish with compressed retinotectal projections. Neuroscience 6:697–702
Meek J, Schellart NAM (1978) A Golgi study of Goldfish optic tectum. J Comp Neurol 182:89–122
Ostberg A, Norden J (1979) Ultrastructural study of degeneration and regeneration in the amphibian tectum. Brain Res 168:441–456
Parmentier M, Ghysens M, Rypens F, Lawson DEM, Pasteels JL, Pochet R (1987) Calbindin in vertebrate classes: immunohistochemical localization and western blot analysis. Gen Comp Endocrinol 65:399–407
Pontet A, Danger JM, Dubourg P, Pelletier G, Vaudry H, Calas A, Kah O (1989) Distribution and characterization of neuropeptide Y-like immunoreactivity in the brain and pituitary of goldfish. Cell Tissue Res 255:529–538
Schreiner DS, Jande SS, Lawson DEM (1985) Target cells of vitamin D in the vertebrate retina. Acta Anat 121:153–162
Thomasset M, Parkes CO, Cuisinier-Gleizes P (1982) Rat calcium-binding proteins: distribution, development and vitamin D. Am J Physiol 243:E483-E488
Vecino E, Ekstrom P (1990) Distribution of met-enkephalin, leuenkephalin, substance P, neuropeptide Y, FMRFamide, and serotonin immunoreactivities in the optic tectum of the Atlantic salmon (Salmo salar L.). J Comp Neurol 299:229–241
Zamboni L, De Martino C (1967) Buffered picric acid formaldehyde: a new rapid fixative for electron microscopy. J Cell Biol 35:148A
Zottoli SJ, Rhodes KJ, Mufson EJ (1987) Comparison of acetyl-cholinesterase and choline acetyltransferase staining patterns in the optic tectum of the goldfish Carassius auratus. Brain Behav Evol 30:143–159
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Miguel-Hidalgo, J.J., Ito, H. & Lara, J. Distribution of calbindinlike immunoreactive structures in the optic tectum of normal and eye-enucleated cyprinid fish. Cell Tissue Res 265, 511–516 (1991). https://doi.org/10.1007/BF00340874
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DOI: https://doi.org/10.1007/BF00340874