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The functional and structural homology of ribosomal protein S7 of E. coli strains K and MRE600

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Summary

The 30S ribosomal protein S7 purified from E. coli MRE600 displaces specifically and stoichiometrically the endogenous K-S7 protein when it is added to a reconstitution system containing total K strain 30S protein and 16S RNA. The S7 proteins from the two strains have been shown to contain a group of common trypic peptides and to crossreact immunologically. Therefore, the 30S ribosomal protein S7 from E. coli K strain and MRE600 are functionally and structurally homologous despite differences in amino acid composition, molecular weight and electrophoretic mobility.

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References

  • Birge, E. A., Craven, G. R., Hardy, S. J. S., Kurland, C. G., Voynow, P.: Structural determinant of a ribosomal protein: K locus. Science 164, 1285–1286 (1969).

    Google Scholar 

  • Hill, W. E., Rossetti, G. P., Holde, K. E. van: Physical studies of ribosomes from Escherichia coli. J. molec. Biol. 44, 263–277 (1969).

    Google Scholar 

  • Kaltschmidt, E., Stöffer, G., Dzionara, M., Wittmann, H. G.: Ribosomal proteins. XVII Comparative studies on ribosomal proteins of four strains of Escherichia coli. Molec. gen. Genet. 109, 303–308 (1970).

    Google Scholar 

  • Kurland, C. G.: Molecular characterization of ribonucleic acid from Escherichia coli ribosomes. I. Isolation and molecular weights. J. molec. Biol. 2, 83–91 (1960).

    Google Scholar 

  • Leboy, P. S., Cox, E. C., Flaks, J. G.: The chromosomal site specifying a ribosomal protein. Proc. nat. Acad. Sci. (Wash.) 52, 1367–1374 (1964).

    Google Scholar 

  • Mayuza, C., Meier, D., Wang, T.: Escherichia coli: the K12 ribosomal protein and the streptomycin region of the chromosome. Biochem. biophys. Res. Commun. 33, 203–206 (1968).

    Google Scholar 

  • Mizushima, S., Nomura, M.: Assembly mapping of 30S ribosomal protein from E. coli. Nature (Lond.) 226, 1214–1218 (1970).

    Google Scholar 

  • Moore, P. B., Traut, R. R., Noller, H., Pearson, P., Delius, D.: Ribosomal proteins of Escherichia Coli. II. Proteins from the 30S subunit. J. molec. Biol. 31, 441–461 (1968).

    Google Scholar 

  • Nomura, M., Traub, P., Bechmann, H.: Hybrid 30S ribosomal particles reconstituted from components of different bacterial origins. Nature (Lond.) 219, 793–799 (1968).

    Google Scholar 

  • Osawa, S., Itoh, T., Otaka, E.: Differentiation of the ribosomal protein compositions in the genus Escherichia and its related bacteria. J. Bact. 107, 168–178 (1971).

    Google Scholar 

  • Osawa, S., Takata, R., Dekio, S.: Genetic studies of the ribosomal proteins in Escherichia coli. III. Compositions of ribosomal proteins in various strains of Escherichia coli. Molec. gen. Genet. 107, 32–38 (1970).

    Google Scholar 

  • Sun, T. T., Bickle, T. A., Traut, R. R.: Similarity in the size and number of ribosomal proteins from different prokaryotes. J. Bact. 111, 474–480 (1972).

    Google Scholar 

  • Sypherd, P.: Amino acid differences in a 30S ribosomal protein from two strains of Escherichia coli. J. Bact. 99, 379–382 (1969).

    Google Scholar 

  • Traub, P., Mizushima, S., Lowry, C. V., Nomura, M.: Reconstitution of ribosomes from subribosomal components. In: Methods in enzymology, eds. Moldave, K. and Grossman, L. vol. XX, p. 391–407. New York-London: Academic Press 1971.

    Google Scholar 

  • Traub, P., Nomura, M.: Structure and function of Escherichia coli ribosomes. VI. Mechanism of assembly of 30S ribosomes studied in vitro. J. molec. Biol. 40, 391–413 (1969).

    Google Scholar 

  • Traut, R. R.: Acrylamide gel electrophoresis of radioactive ribosomal protein. J. molec. Biol. 21, 571–576 (1966).

    Google Scholar 

  • Traut, R. R., Delius H, Ahmad-Zadeh, C., Bickle, T. A., Pearson, P., Tissieres, A.: Ribosomal proteins of E. coli: Stoichiometry and implications for ribosome structure. Cold Spr. Harb. Symp. quant. Biol. 34, 25–38 (1969).

    Google Scholar 

  • Voynow, P., Kurland, C. G.: Stoichiometry of the 30S ribosomal proteins of Escherichia coli. Biochemistry 10, 517–524 (1971).

    Google Scholar 

  • Weber, K., Osborn, M.: The reliability of molecular weight determination by dodecylsulphate polyacrylamide gels. J. biol. Chem. 244, 4406–4412 (1969).

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Chemistry, School of Medicine, University of California, Davis, California, USA

    Tung-Tien Sun & Robert R. Traut

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  1. Tung-Tien Sun
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  2. Robert R. Traut
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Communicated by M. Nomura

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Sun, TT., Traut, R.R. The functional and structural homology of ribosomal protein S7 of E. coli strains K and MRE600. Molec. gen. Genet. 122, 1–9 (1973). https://doi.org/10.1007/BF00337968

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  • DOI: https://doi.org/10.1007/BF00337968

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