Abstract
Oxalate crystals and elements binding to the surfaces of mycorrhizal fungal hyphae were examined using scanning electron microscopy coupled with X-ray analysis of elemental composition. Mycorrhizae from the arid zone vegetation types in southern California were examined including chaparral, riparian oak woodlands, coastal sage, grasslands, and deserts. Only mat-forming ectomycorrhizal hyphae, such as Hysterangium separabile, were found to produce oxalate crystals. None of the arbuscular mycorrhizal fungal hyphae (Glomus spp. and Acaulospora elegans) examined had crystal structures associated with them. The hyphae of Hysterangium separabile without crystals did not show the Ca peaks that were present when the crystals existed nor did the arbuscular mycorrhizal fungal hyphae have the Ca peaks. The elimination of arbuscular mycorrhizae using benomyl did not affect soil P or oxalate. These data indicate that there are some fundamental differences in chemical exudation between mycorrhizal fungi that could affect P uptake and cycling in arid ecosystems.
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References
Allen EB, Allen MF (1988) Facilitation of succession by the nonmycotrophic colonizer Salsola kali (Chenopodiaceae) on a harsh site: effects of mycorrhizal fungi. Am J Bot 75:257–266
Allen MF (1991) The ecology of mycorrhizae. Cambridge University Press, London
Allen MF (1993) Microbial and phosphate dynamics in a restored shrub steppe in southwestern Wyoming. Restoration Ecol 1:196–205
Allen MF, Sexton JC, Moore TS Jr, Christensen M (1981) Influence of phosphorus source on vesicular-arbuscular mycorrhizae of Bouteloua gracilis. New Phytol 87:687–694
Black CH (1987) Biomass, nitrogen, and phosphorus accumulation over a southern California fire cycle chronosequence. In: Tenhunen JD, Catarino FM, Lange OL, Oechel WC (eds) Plant response to stress-functional analysis in Mediterranean ecosystems. Springer, Berlin Heidelberg New York, Berlin, pp 445–458
Boyetchko SM, Tewari JP (1986) A new species of Glomus (Endogonaceae, Zygomycotina) mycorrhizal with barley in Alberta. Canad J Bot 64:90–95
Cannon JP, Allen EB, Allen MF, Dudley LM, Jurinak JJ (1995) The effects of oxalates produced by Salsola tragus on the phosphorus nutrition of Stipa pulchra. Oecologia (Berlin) 102:265–272
Cromack K Jr, Sollins KP, Graustein WC, Speidel K, Todd AW, Spyker G, Li CY, Todd RL (1979) Calcium oxalte accumulation and soil weathering in mats of the hypogeous fungus Hysterangium crassum. Soil Biol Biochem 11:463–468
Fitter AH, Nichols R (1988) The use of benomyl to control infection by vesicular-arbuscular mycorrhizal fungi. New Phytol 110:201–206
Fox TR, Comerford NB (1990) Low molecular weight organic acids in selected forest soils of the Southeastern USA. Soil Sci Soc Am J 554:1139–1144
Fox TR, Comerford NB (1992) Influence of oxalate loading on phosphorus and aluminum solubility in spodosols. Soil Sci Soc Amer J 56:290–294
Graustein VC, Cromack K, Sollins P (1977) Calcium oxalate: occurrence in soils and effect on nutrient and geochemical cycles. Science 198:1252–1254
Hanes TT (1971) Succession after fire in the chaparral of Southern California. Ecol Monogr 41:27–52
Hartnett DC, Samenus RJ, Fischer LE, Hetrick BAD (1994) Plant demographic responses to mycorrhizal symbiosis in tallgrass prairie. Oecologia (Berlin) 99:21–26
Jurinak JJ, Dudley LM, Allen MF, Knight WG (1986) The role of calcium oxalate in the availability of phosphorus in soils of semiarid regions: a thermodynamic study. Soil Sci 142:255–261
Keeley JE (1992) Recruitment of seedlings and vegetative sprouts in unburned chaparral. Ecology 73:1194–1208
Knight WG, Allen MF, Jurinak JJ, Dudley LM (1989) Elevated carbon dioxide and solution phosphorus in soil with vesicular-arbuscular mycorrhizal western wheatgrass. Soil Sci Soc AM J 53:1075–1082
Knight WG, Dudley LM, Jurinak JJ (1992) Oxalate effects on solution phosphorus in a calcareous soil. Arid Soil Res Rehabil 6:11–20
Lapeyrie F, Perrin M, Pepin R, Bruchet G (1984) Formation de weddellite (CaC2O4 2 H2O) extracellulaire en culture in vitro par Paxillus involutus: signification de cette production pour la symbiose ectomycorrhizienne. Canad J Bot 62:1116–1121
Lilieholm BC, Dudley LM, Jurinak JJ (1992) Oxalate determination of soils using ion chromatography. Soil Sci Soc Am J 56:324–326
Malajczuk N, Cromack K Jr (1982) Accumulation of calcium oxalate in the mantle of ectomycorrhizal roots of Pinus radiata and Eucalyptus marginata. New Phytol 65:527–531
Mouton M, Wingfield MJ, Vanwyk PS (1992) The anamorph of Ophiostoma francke-grosmanniae is a Leptographium. Mycologia 84:857–862
Page AL, Miller RH, Keeney DR (eds) (1982) Methods of soil analysis, part 2. Chemical and microbiological properties, 2nd edn. American Society of Agronomy, Soil Science Society of America. Madison, WI
Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea: how can it occur? Biogeochem 13:87–115
Weinbaum BS (1993) Tracking the fate of Acaulospora elegans and Scutellospora calospora transplanted between two distant sagebrush populations. MS Thesis, San Diego State University. San Diego, CA
Whitney K, Arnott HJ (1986) Calcium oxalate crystals and basidiocarp dehiscence in Geastrum saccatum (Gasteromycetes). Mycologia 78:649–656
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Allen, M.F., Figueroa, C., Weinbaum, B.S. et al. Differential production of oxalate by mycorrhizal fungi in arid ecosystems. Biol Fertil Soils 22, 287–292 (1996). https://doi.org/10.1007/BF00334571
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DOI: https://doi.org/10.1007/BF00334571