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Cloning and characterization of the ribosomal RNA genes of Rhynchosciara americana

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Abstract

The ribosomal RNA genes (rDNA) of Rhynchosciara americana were analysed using Southern transfers of DNA cleaved with EcoRI, HindIII, BamHI and PstI. The results show that the rDNA is heterogeneous in structure. Following digestion with EcoRI and hybridization to rRNA three bands corresponding to fragments of 9.5, 7.5 and 5.5 kilobases (kb) were detected. Recombinants containing EcoRI fragments of R. americana DNA were prepared using the vector λgtλB. Three different recombinants (λgtRa1, λgtRa23 and λgtRa5) were isolated containing the rDNA fragments of 9.5, 7.5 and 5.5 kb, respectively. These fragments were transferred to pBR325 and analysed with restriction enzymes and Southern hybridization with 28 S and 18 S rRNA. The λgt recombinants were further analysed by R-loop mapping. The data show that the rDNA occurs in two different repeating gene units. A shorter repeat of 9.5 kb and a longer repeat of 13 kb, in which the 28 S rRNA coding sequence contains an insertion of 3.5 kb.

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Author notes

  1. Arnaldo Zaha

    Present address: Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS

  2. Orilio Leoncini

    Present address: Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil

  3. Cornelis P. Hollenberg

    Present address: Institut für Mikrobiologie, Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf, Federal Republic of Germany

Authors and Affiliations

  1. Max-Planck-Institut für Biologie, Abteilung W. Beermann, Spemannstrasse 34, D-74, Tübingen, Federal Republic of Germany

    Arnaldo Zaha, Orilio Leoncini, Cornelis P. Hollenberg & Francisco J. S. Lara

  2. Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, Brasil

    Arnaldo Zaha, Orilio Leoncini, Cornelis P. Hollenberg & Francisco J. S. Lara

Authors
  1. Arnaldo Zaha
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  2. Orilio Leoncini
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  3. Cornelis P. Hollenberg
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  4. Francisco J. S. Lara
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Zaha, A., Leoncini, O., Hollenberg, C.P. et al. Cloning and characterization of the ribosomal RNA genes of Rhynchosciara americana . Chromosoma 87, 103–116 (1982). https://doi.org/10.1007/BF00333512

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  • DOI: https://doi.org/10.1007/BF00333512

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