Abstract
The fission yeast Schizosaccharomyces pombe has a haplontic life cycle in which the diplophase is confined to the zygote. Through the use of one- and two-step protoplast fusions we show that the ploidy can be increased up to pentaploid. The polyploid fusion products are rather unstable and segregate cells of lower ploidies by gradual loss of chromosomes during mitotic divisions. The polyploid cells conjugate normally but are prone to arrest at various stages of meiosis (1-, 2- and 3-spored asci, binucleate spores) and/or produce inviable, most probably aneuploid, spores. Marker segregation in the complete tetrads indicates the multiple association of homologous chromosomes. In tetra- and penta-ploid meiosis, multispored (6- to 7-spored) asci are also produced, probably by postmeiotic division of the nuclei.
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References
Egel R (1989) Mating-type genes, meiosis, and sporulation. In: Nasim A, Young P, Johnston BF (eds) Molecular biology of the fission yeast. Academic Press, New York, pp 31–73
Flores da Cunha M (1970) Genet Res 16:127–144
Grallert B, Sipiczki M (1989) Curr Genet 15:231–233
Grallert B, Sipiczki M (1991) Curr Genet 20:199–204
Gunge N (1966) Jpn J Genet 4:203–214
Gutz H (1967) Science 158:796–798
Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RC (ed) Handbook of genetics. Plenum Press, New York, pp 395–446
Hirata A, Tanaka K (1982a) J Cell Sci 56:263–279
Hirata A, Tanaka K (1982b) J Gen Appl Microbiol 28:263–274
Kielland-Brandt MC, Nilsson-Tillgren T, Petersen JGL, Holmberg S, Gjermansen C (1983) Approaches to the genetic analysis and breeding of brewer's yeast. In: Spencer JFT, Spencer DM, Smith ARW (eds) Yeast genetics. Fundamental and applied aspects. Springer Verlag, New York, pp 421–437
Leupold U (1955) Schweiz Z Allg Pathol Bakteriol 18:1141–1146
Leupold U (1956) C R Trav Lab Carlsberg, Ser Physiol 26:221–251
Mortimer RK (1958) Radiat Res 9:312–326
Moreno S, Klar A, Nurse P (1991) Methods Enzymol 194:795–823
Munz P, Wolf K, Kohli J, Leupold U (1989) Genetics overview. In: Nasim A, Young P, Johnston BF (eds) Molecular biology of the fission yeast. Acedemic Press, New York, pp 1–30
Nakaseko Y, Niwa O, Yanagida M (1984) J Bacteriol 157:334–336
Niwa O, Yanagida M (1985) Curr Genet 9: 463–470
Sipiczki M (1989) Taxonomy and phylogenesis. In: Nasim A, Young P, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, New York, pp 431–452
Sipiczki M, Ferenczy L (1977) Mol Gen Genet 151:77–81
Sipiczki M, Heyer W-D, Kohli J (1985) Curr Microbiol 12:169–174
Takagi A, Harashima S, Ishima Y (1983) Appl Environ Microbiol 45:1034–1040
Thomas JH, Botstein D (1986) Cell 44:65–76
Whittaker SG, Rockmill BM, Blechl AE, Maloney DH, Resnick MA, Fogel S (1988) Mol Gen Genet 215:10–18
Widra A, DeLamater ED (1955) Am J Bot 42:423–435
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Communicated by K. Wolf
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Molnar, M., Sipiczki, M. Polyploidy in the haplontic yeast Schizosaccharomyces pombe: construction and analysis of strains. Curr Genet 24, 45–52 (1993). https://doi.org/10.1007/BF00324664
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DOI: https://doi.org/10.1007/BF00324664