Abstract
We have used immunohistochemistry and electron microscopy to investigate the distribution of basic fibroblast growth factor (bFGF) in the peripheral motor system of the adult rat. In the lumbar segments of the spinal cord, bFGF immunoreactivity (bFGF-I) was seen in motor neurons and glial cells but not in axons. The neuronal immunostaining was seen as two or three intensely fluorescent spots in the nuclei with weak and more diffuse staining of the perinuclear cytoplasm. In the sciatic nerve, bFGF-I was seen in Schwann cells with strong intensity at the nodes of Ranvier. Axonal immunostaining could not be detected. At the electron microscopic level, the intense nodal immunostaining of Schwann cells was confirmed and was found to be localized to the Schwann cell membrane at the nodal gap. The intensity of staining decreased with distance from the node. In the soleus and gastrocnemius muscles, bFGF-I was seen at the motor endplates in sites corresponding to the motor nerve terminals in addition to faint staining within the muscle fibers. Electron microscopy showed that bFGF-I was localized within the nerve terminals. Histochemical localization of bFGF in the peripheral motor system is compatible with the functions ascribed for this protein in this system.
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References
Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction products. J Histochem Cytochem 29: 775–780
Arkawa Y, Sendtner M, Thoenen H (1990) Survival effects of ciliary neurotrophic factor (CNTF) on chick embryonic motoneurons in culture: comparison with other neurotrophic factors and cytokines. J Neurosci 10: 3507–3515
Davis JB, Stroobant P (1990) Platelet-derived growth factors and fibroblast growth factors are mitogens for rat Schwann cells. J Cell Biol 110: 1353–1360
DiMario J, Buffinger N, Yamada S, Strohman RC (1989) Fibroblast growth factor in the extracellular matrix of dystrophic (mdx) mouse muscle. Science 244: 688–690
Eckenstein FP, Esch F, Holbert T, Blacher RW, Nishi R (1990) Purification and characterization of a trophic factor for embryonic peripheral neurons: comparison with fibroblast growth factors. Neuron 4: 623–631
Ferguson IA, Schweitzer JB, Johnson EM (1990) Basic fibroblast growth factor: receptor-mediated internalization, metabolism, and anterograde axonal transport in retinal ganglion cells. J Neurosci 10: 2176–2189
Goldfarb M (1990) The fibroblast growth factor family. Cell Growth Differ 1: 439–445
Grothe C, Unsicker K (1992) Basic fibroblast growth factor in the hypoglossal system: specific retrograde transport, trophic, and lesion-related responses. J Neurosci 32: 317–328
Grothe C, Zachman K, Unsicker K (1991) Basic FGF-like immunoreactivity in the developing and adult rat brain stem. J Comp Neurol 305: 328–336
Gurney ME, Yamamoto H, Kwon Y (1992) Induction of motor neuron sprouting in vivo by ciliary neurotrophic factor and basic fibroblast growth factor. J Neurosci 12: 3241–3247
Hatten ME, Lynch M, Rydel RE, Sanchez J, Joseph-Silverstein J, Moscatelli D, Rifkin DB (1988) In vitro neurite extension by granule neurons is dependent upon astroglial-derived fibroblast growth factor. Dev Biol 81: 6963–6967
Kerkhoff H, Hassan SM, Troost D, Van Etten RW, Veldman H, and Jennekens FGI (1994) Insulin-like and fibroblast growth factors in spinal cords, nerve roots and skeletal muscle of human controls and patients with amyotrophic lateral sclerosis. Acta Neuropathol 87: 411–421
Matsuda S, Desaki J, Okumura N, Shiosaka S, Imaoka S, Sakanaka M (1992) Basic fibroblast growth factor-like immunoreactivity in the trigeminal proprioceptive and motor systems. Brain Res 577: 92–100
Matsuda S, Desaki J, Fujita H, Okumura N Sakanaka M (1992) Immuno-electron microscopic localization of basic fibroblast growth factor in the dystrophic mdx mouse masseter muscle. Cell Tissue Res 270: 569–576
McManaman J, Crawford F, Clark P, Richker J, Fuller F (1989) Multiple neurotrophic factors from skeletal muscle: demonstration of effects of basic fibroblast growth factor and comparisons with the 22-kilodalton choline acetyltransferase development factor. J Neurochem 53: 1763–1771
McManaman JL, Haverkamp LJ, Oppenheim RW (1991) Skeletal muscle proteins rescue motor neurons from cell death in vivo. Adv Neurol 56: 81–88
Oppenheim RW, Prevette D, Fuller F (1992) The lack of effect of basic fibroblast growth factors on the naturally occurring death of motoneurons in the chick embryo. J Neurosci 12: 2726–2736
Otto D, Unsicker K, Grothe C (1987) Pharmacological effects of nerve growth factor and fibroblast growth factor applied to the transected sciatic nerve on neuron death in adult rat dorsal root ganglia. Neurosci Lett 83: 156–160
Peng HB, Baker LP, Chen Q (1991) Induction of synaptic development in cultured muscle cells by basic fibroblast growth factor. Neuron 6: 237–246
Pettman B, Weibel M, Sensenbrenner M, Labourdette G (1985) Purification of two astroglial growth factors from bovine brain. FEBS Lett 189: 102–108
Unsicker K, Reichert-Preibsch H, Schmidt R, Labourdette G, Sensenbrenner M (1987) Acidic and basic fibroblast growth factors have neurotrophic functions for cultured peripheral and central nervous system neurons. Proc Natl Acad Sci USA 84: 5459–5463
Wagner JA (1991) The fibroblast growth factors: an emerging family of neural growth factors. Curr Top Microbiol Immunol 165: 95–118
Wallicke PA, Baird A (1991) Internalization and processing of basic fibroblast growth factor by neurons and astrocytes. J Neurosci 11: 2249–2258
Wanaka A, Johnson EM, Milbrandt J (1990) Localization of FGF receptor mRNA in the adult rat central nervous system by in situ hybridization. Neuron 5: 267–281
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Hassan, S.M., Kerkhoff, H., Troost, D. et al. Basic fibroblast growth factor immunoreactivity in the peripheral motor system of the rat. Acta Neuropathol 87, 405–410 (1994). https://doi.org/10.1007/BF00313610
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DOI: https://doi.org/10.1007/BF00313610