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Immunogold labelling of paired helical filaments and amyloid fibrils by specific monoclonal and polyclonal antibodies

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Abstract

Senile plaque and paired helical filament (PHF) formation are characteristic of Alzheimer's disease, but the mechanisms leading to these lesions still remain unclear. To understand them better, we have performed different immunolabellings of amyloid protein and PHF. We describe a very specific immunodetection of PHF with AD2, a monoclonal antibody directed against a hyperphosphorylated epitope of PHF-tau, and use double immunolabelling to show that PHF and plaque amyloid are discretely labbeled by different antibodies. We also discuss different mechanisms of PHF maturation.

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Authors and Affiliations

  1. INSERM Unité 422, 1 Place de Verdun, F-59045, Lille Cedex, France

    S. Reig, V. Buée-Scherrer, A. Defossez, A. Delacourte, J. C. Beauvillain & M. Mazzuca

  2. CNRS/9921 UFR Pharmacie, Université de Montpellier I, F-34060, Montpellier Cedex, France

    C. Mourton-Gilles

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  1. S. Reig
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  2. V. Buée-Scherrer
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  3. A. Defossez
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  4. A. Delacourte
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  5. J. C. Beauvillain
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  6. M. Mazzuca
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  7. C. Mourton-Gilles
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Reig, S., Buée-Scherrer, V., Defossez, A. et al. Immunogold labelling of paired helical filaments and amyloid fibrils by specific monoclonal and polyclonal antibodies. Acta Neuropathol 90, 441–447 (1995). https://doi.org/10.1007/BF00294803

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  • DOI: https://doi.org/10.1007/BF00294803

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