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Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids

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Summary

A set of plasmids that contain fragments of the bacteriophage P4 genome has been constructed by deleting portions of a P4-ColE1 hybrid. A P4 genetic map has been established and related to the physical map by examining the ability of these plasmids to rescue various P4 mutations. The P4 vir1 mutation and P4 genes involved in DNA replication (α), activation of P2 helper genes (δ and ε), polarity suppression (psu) and head size determination (sid) have been mapped, as has the region responsible for synthesis of a nonessential P4 protein.

One of the deleted plasmids contains only 5900 base pairs (52%) of P4 but will form plaques if additional DNA is added to increase its total size to near that of P4. This plasmid is also unique in that it will not form stable associations with P2 lysogens of E. coli which are recA +. P4 α mutants can be suppressed as a result of replication under control of the ColE1 part of the hybrid.

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Author notes

  1. Michael Kahn

    Present address: Department of Bacteriology and Public Health, Washington State University, 99164, Pullman, Washington

  2. David Ow

    Present address: The Biological Laboratories, Harvard University, 16 Divinity Avenue, 02138, Cambridge, Massachusetts

  3. Brian Sauer

    Present address: Biochemistry Department, Stanford University, 94305, Stanford, California

Authors and Affiliations

  1. Department of Biology, B-022, University of California at San Diego, 92093, La Jolla, California, USA

    Michael Kahn & Arthur Rabinowitz

  2. Department of Molecular Biology, University of California, 94720, Berkeley, California, USA

    David Ow, Brian Sauer & Richard Calendar

Authors
  1. Michael Kahn
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  2. David Ow
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  3. Brian Sauer
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  4. Arthur Rabinowitz
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  5. Richard Calendar
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Communicated by H.A. Boyer

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Kahn, M., Ow, D., Sauer, B. et al. Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids. Molec. Gen. Genet. 177, 399–412 (1980). https://doi.org/10.1007/BF00271478

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  • DOI: https://doi.org/10.1007/BF00271478

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