Summary
The genetic organization of unc-26(IV) and adjacent regions was studied in Caenorhabditis elegans. We constructed a fine structure genetic map of unc-26(IV), a gene that affects locomotion and pharyngeal muscle movement but not muscle structure. Eleven alleles were positioned relative to each other recombinationally and were classified according to phenotypic severity. The unc-26 gene spans at least 0.026 map units, which is exceptionally large for a C. elegans gene. All but one allele, e205, are amorphic alleles. Interestingly, e205 is hypomorphic but also suppressible by the amber suppressor sup-7. Nineteen lethal mutations in the unc-26 region were isolated and characterized. The unc-26 region is subdivided into four zones by five deficiency breakpoints. These mutations fall into 15 complementation groups. The stages of development affected by these mutations were determined.
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References
Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77:71–94
Bullerjahn AME, Riddle DL (1988) Fine-structure genetics of ama-1, an essential gene encoding the amanitin-binding subunit of RNA polymerase 11 in C. elegans. Genetics 120:423–434
Clark DV, Rogalski TM, Donati LM, Baillie DL (1988) The unc22(IV) region of Caenorhabditis elegans: genetic analysis of lethal mutations. Genetics 119:345–353
Coulson A, Sulston J, Brenner S, Karn J (1986) Toward a physical map of the genome of the nematode C. elegans. Proc Natl Acad Sci USA 83:7821–7825
Edgley ML, Riddle DL (1987) Caenorhabditis elegans. In: Genetic maps 1987, vol 4. O'Brien SJ (ed) Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Ferguson ED, Horvitz HR (1985) Identification and characterization of 22 genes that affect vulval cell lineages of the nematode Caenorhabditis elegans. Genetics 110:17–72
Greenwald I, Coulson A, Sulston J, Priess J (1987) Correlation of the physical and genetic maps in the lin-12 region of C. elegans. Nucleic Acids Res 15:2295–2307
Hodgkin J (1987) A genetic analysis of the sex-determining gene tra-1, in the nematode Caenorhabditis elegans. Genes Dev 1:731–745
Horvitz HR, Brenner S, Hodgkin J, Herman RK (1979) A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Mol Gen Genet 175:128–133
Howell AM, Gilmour SG, Mancebo RA, Rose AM (1987) Genetic analysis of a large autosomal region in Caenorhabditis elegans by the use of a free duplication. Genet Res 49:207–213
McKim KS, Heschl MFP, Rosenbluth RE, Baillie DL (1988) Genetic organization oil the unc-60 region in Caenorhabditis elegans. Genetics 118:49–59
Meneely PM, Herman RK (1979) Lethals, steriles and deficiencies in a region of the X chromosome of Caenorhabditis elegans. Genetics 92:99–115
Moerman DG, Baillie DL (1979) Genetic organization in Caenorhabditis elegans: Fine structure analysis of the unc-22 gene. Genetics 91:95–103
Moerman DG, Baillie DL (1981) Formaldehyde mutagenesis in the nematode Caenorhabditis elegans. Mutat Res 80:273–289
Moerman DG, Waterston RH (1984) Spontaneous unstable unc-22 IV mutations in C. elegans var. Bergerac. Genetics 108:859–877
Mori I, Moerman DG, Waterston RH (1988) Analysis of a mutator activity necessary for germline transposition and excision of Tc1 transposable elements in C. elegans. Genetics 120:397–407
Muller HJ (1932) Further studies on the nature and causes of gene mutations. Proceedings of the Sixth International Congress of Genetics 1:213–255
Rogalski TM, Baillie DL (1985) Genetic organization of the unc-22 IV gene and the adjacent region in Caenorhabditis elegans. Mol Gen Genet 201:409–414
Rogalski TM, Moerman DG, Baillie DL (1982) Essential genes and deficiencies in the unc-22 IV region of Caenorhabditis elegans. Genetics 102:725–736
Rose AM, Baillie DL (1979) The effect of temperature and parental age on recombination and nondisjunction in Caenorhabditis elegans. Genetics 92:409–418
Rose AM, Baillie DL (1980) Genetic organization of the region around unc-151, a gene affecting paramyosin in Caenorhabditis elegans. Genetics 196:639–648
Rosenbluth RE, Rogalski TM, Johnsen RC, Addison LM, Baillie DL (1988) Genomic organization in Caenorhabditis elegans: deficiency mapping on linkage group IV (left). Genet Res 52:105–118
Stevens WL (1942) Accuracy of mutation rates. J Genet 43:301–307
Waterston RH (1981) A second informational suppressor, sup-7X, in Caenorhabditis elegans. Genetics 97:307–325
Waterston RH, Thomson JN, Brenner S (1980) Mutants with altered muscle structure in Caenorhabditis elegans. Dev Biol 77:271–302
Waterston RH, Smith KC, Moerman DG (1982) Genetic fine-structure analysis of the myosin heavy chain gene, unc-54, of Caenorhabditis elegans. J Mol Biol 158:1–15
White JG, Southgate E, Thomson JN, Brenner S (1976) The structure of the ventral nerve cord of C. elegans. Philos Trans R See Lon 275B:327–348
White JG, Horvitz HR, Sulston JE (1982) Neurone differentiation in cell lineage mutants of Caenorhabditis elegans. Nature 297:584–593
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Communicated by B,H. Judd
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Charest, D.L., Clark, D.V., Green, M.E. et al. Genetic and fine structure analysis of unc-26(IV) and adjacent regions in Caenorhabditis elegans . Mol Gen Genet 221, 459–465 (1990). https://doi.org/10.1007/BF00259412
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DOI: https://doi.org/10.1007/BF00259412