Abstract
Constitutive heterochromatin of a karyotypically conserved species of harvest mouse was compared to that of three karyotypically derived species of harvest mice by examining banding patterns produced on metaphase chromosomes with three restriction endonucleases (EcoRI, MboI and PstI). Banding patterns produced by two of these restriction endonucleases (EcoRI and MboI) were compared to published G- and C-banded karyotypes and in situ hybridization of a satellite DNA repeat for these taxa. The third restriction endonuclease (PstI) did not produce a detectable pattern of digestion. For the most part, patterns produced by EcoRI and MboI can be related to C-banded chromosomes and in situ hybridization of satellite DNA sequences. Moreover, digestion with EcoRI reveals bands not apparent with these other techniques, suggesting that restriction endonuclease digestion of metaphase chromosomes may provide additional insight into the structure and organization of metaphase chromosomes. The patterns produced by restriction endonuclease digestion are compatible with the chromosomal evolution of these taxa, documenting that in the highly derived taxa not only are the chromosomes rearranged but the abundance of certain sequences is highly variable. However, technical variation and difficulty in producing consistent results even on a single slide with some restriction endonucleases documents the problems associated with this method.
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Arnold, M. L., L. W.Robbins, R. K.Chesser & J. C.Patton, 1983. Phylogenetic relationships among six species of Reithrodontomys. J. Mamm. 64: 128–132.
Babu, A., 1988. Heterogeneity of heterochromatin of human chromosomes as demonstrated by restriction endonuclease treatment, Pp. 250–275 in Heterochromatin: Molecular and structural aspects, edited by R. S.Verma. Cambridge University Press. Cambridge, England.
Babu, A. & R. S.Verma, 1986. Expression of heterochromatin by restriction endonuclease treatment and distamycin A/DAPI staining of India muntjac (Muntiacus muntjak) chromosomes. Cytogenet. Cell Genet. 41: 96–100.
Bianchi, N. O., M. S.Bianchi & J. E.Cleaver, 1984. The action of ultraviolet light on the pattern of banding induced by restriction endonucleases in human chromosomes. Chromosoma 90: 133–138.
Bianchi, M. S., N. O.Bianchi, G. E.Pantelias & S.Wolff, 1985a. The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes. Chromosoma 91: 131–136.
Bianchi, N. O., M. S.Bianchi, J. E.Cleaver & S.Wolff, 1985b. The pattern of restriction enzyme-induced banding in the chromosomes of Chimpanzee, Gorilla, and Orangutan and its evolutionary significance. J. Mol. Evol. 90: 323–333.
Britten, R. J. & D. E.Kohne, 1968. Repeated sequences in DNA. Science 161: 529–540.
Burkholder, G., 1989. Morphological and biochemical effects of endonucleases on isolated mammalian chromosomes in vitro. Chromosoma 97: 347–355.
Comings, D. E., 1978. Mechanisms of chromosome banding and implications for chromosome structure. Ann. Rev. Genet. 12: 25–46.
Gosalvez, J., C.Lopez-Fernandez, L.Ferrucci & R.Mezzanotte, 1989. DNA base sequence is not the only factor for restriction endonuclease activity on metaphase chromosomes: evidence using isoschizomers. Cytogenet. Cell Genet. 50: 142–144.
Hamilton, M. J., R. L.Honeycutt & R. J.Baker, 1990. Intragenomic movement, sequence amplification and concerted evolution in satellite DNA in harvest mice, Reithrodontomys: evidence from in situ hybridization. Chromosoma 99: 321–329.
Hood, C. S., L. W.Robbins, R. J.Baker & H. S.Shellhammer, 1984. Chromosomal studies and evolutionary relationships of an endangered species, Reithrodontomys raviventris. J. Mamm. 65: 655–667.
Hsu, T. C., 1973. Longitudinal differentiation of chromosomes. Ann. Rev. Genet. 7: 153–156.
John, B., 1988. The biology of heterochromatin. Pp. 1–147 in Heterochromatin: Molecular and structural aspects (R. S.Verma ed.). Cambridge University Press. Cambridge, England.
Lee, M. R. & F. F. B.Elder, 1980. Yeast stimulation of bone marrow mitosis for cytogenetic investigation. Cytogenet. Cell Genet. 26: 36–40.
Lica, L. & B.Hamkalo, 1983. Preparation of centromeric heterochromatin by restriction endonuclease digestion of mouse L929 cells. Chromosoma 88: 42–49.
Lima-De-Faria, A., M.Isaksson & E.Olsson, 1980. Action of restriction endonucleases on the DNA and chromosomes of Muntiacus muntjak. Hereditas 92: 267–273.
Mezzanotte, R., U.Bianchi, R.Vanni & L.Ferrucci, 1983. Chromatin organization and restriction endonuclease activity on human metaphase chromosomes. Cytogenet. Cell Genet. 36: 562–566.
Mezzanotte, R. & L.Ferrucci, 1983. The metaphase banding pattern produced in the chromosomes of Culiseta longiareolata (Diptera: Culicidae) by AluI restriction endonuclease or actinomyci-D treatment followed by acridine-orange staining. Genetica 62: 47–50.
Mezzanotte, R. & L.Ferrucci, 1984. Alterations induced in mouse chromosomes by restriction endonucleases. Genetica 64: 123–128.
Mezzanotte, R., L.Ferrucci, R.Vanni & A. T.Sumner, 1985. Some factors affecting the action of restriction endonu-cleases on human metaphase chromosomes. Exp. Cell Res. 161: 247–253.
Miller, D. A., J.Choi & O. J.Miller, 1983. Chromosome localization of highly repetitive DNAs and amplified ribosomal DNA with restriction enzymes. Science 219: 395–397.
Robbins, L. W. & R. J.Baker, 1980. G- and C-band studies on the primitive karyotype for Reithrodomomys. J. Mamm. 61: 708–714.
Singer, M. F., 1982. Highly repeated sequences in mammalian genomes. Int. Rev. Cyt. 76: 67–112.
Sumner, A. T., 1990. Chromosome banding. Unwin Hyman. London. 434 pp.
Zhang, S.-F. & W.-F.Dong, 1989. Human chromosome banding with restriction endonucleases HaeIII, HinfI, and PstI. Sci. in China, 32: 1349–1360.
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Van Den Bussche, R.A., Honeycutt, R.L. & Baker, R.J. Restriction endonuclease digestion patterns of harvest mice (Reithrodontomys) chromosomes: a comparison to G-bands, C-bands, and in situ hybridization. Genetica 87, 141–149 (1993). https://doi.org/10.1007/BF00240553
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DOI: https://doi.org/10.1007/BF00240553