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Evidence for the operation of a cyanide-sensitive oxidase in chlororespiration in the thylakoids of the chlorophyll c-containing alga Pleurochloris meiringensis (Xanthophyceae)

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Abstract

For characterisation of chlororespiration in the chlorophyll c-containing alga Pleurochloris meiringensis, we measured the flash-induced electrochromic absorbance changes between 470 and 545 nm and the redox changes of cytochrome f and cytochrome c 553. Cytochrome c 553 was shown to be present in high amounts (1 mol cytochrome c 553 per 300 mol chlorophyll) in this alga and to function as the obligate electron donor for photosystem I instead of plastocyanin. Whereas salicylhydroxamic acid had no effect on the flash-induced absorbance transients, cyanide enhanced the slow-rising (t1/2≈10 ms) kinetic component of the electrochromic absorbance change. Cyanide also accelerated the re-reduction of the cytochrome f +/c +553 electron pool following the photooxidation by repetitive single-turnover flashes. These data suggest that an oxidase competes with the cytochromes for electrons. The KCN concentration needed to induce these effects was 0.25 mM at half-saturation, whereas mitochondrial respiration was completely blocked at 0.1 mM. Therefore, the oxidase cannot be identical to the cytochrome aa3-oxidase of mitochondria and is most likely located in the chloroplast of P. meiringensis.

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Abbreviations

ΔA520 :

flash-induced electrochromic absorbance change

ΔAcyt(f+c 553):

flash-induced absorbance changes of cyt f and cyt c 553

Chl:

chlorophyll

cyt:

cytochrome

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

PQ:

plastoquinone

SHAM:

salicylhydroxamic acid

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Authors and Affiliations

  1. Institute of General Botany, University of Mainz, D-55099, Mainz, Germany

    Claudia Büchel

  2. Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, H-6701, Szeged, Hungary

    Gyözö Garab

Authors
  1. Claudia Büchel
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  2. Gyözö Garab
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Additional information

The authors kindly acknowledge a grant for C. Büchel from the Deutsche Forschungsgemeinschaft. This work was also supported by a grant from the Hungarian Research Fund. We are grateful to Dr. Wilhelm for fruitful discussion of the manuscript.

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Büchel, C., Garab, G. Evidence for the operation of a cyanide-sensitive oxidase in chlororespiration in the thylakoids of the chlorophyll c-containing alga Pleurochloris meiringensis (Xanthophyceae). Planta 197, 69–75 (1995). https://doi.org/10.1007/BF00239941

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  • DOI: https://doi.org/10.1007/BF00239941

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