Summary
In an attempt to study further the stellate cell and its functions, the ultrastructure of this cell type in the neurointermediate lobe of the bullfrog, Rana catesbeiana, was examined in both organ and dissociated-cell culture. The cytoplasmic activity of stellate cells from neurointermediate lobes incubated 3 1/2 or 5 1/2 h was greater than that of those in vivo. Mitochondria and bundles of cytoplasmic filaments were numerous, in addition to prominent, well-developed Golgi complexes with associated vesicles. The most striking ultrastructural feature was the presence of phagocytic vacuoles that contain cellular debris. The stellate cells were seen to form cytoplasmic processes that phagocytosed this extracellular debris identifiable as belonging to the secretory cells of the pars intermedia. The stellate cells from the dissociated-cell preparations were also seen to contain debris within phagocytic vacuoles. In those neurointermediate lobes transplanted for 3 1/2 to 4 days into the anterior chamber of the eye, the stellate cells demonstrated similar phagocytic ability, but the phagocytic vacuoles contained material that seemed to be at a later stage of degradation. In all three of these conditions, the stellate cells were not seen to release this cellular debris nor were they seen to undergo cell division. These glial-like stellate cells of the pars intermedia acted as macrophages in all three of these experiments. There is now, therefore, a need to determine under what conditions, if any, these stellate cells function in vivo as macrophages.
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References
Båge G, Fernholm B (1975) Ultrastructure of the pro-adenohypophysis of the river lamprey, Lampetra fluviatilis, during gonad maturation. Acta Zool (Stockh) 56:95–118
Brinkley BR, Murphy P, Richardson LC (1967) Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol 35:279–283
Cameron E, Foster CL (1971) Some light- and electron-microscopical observations on the pars intermedia of the pituitary gland of the rabbit. J Endocrinol 49:479–485
Castel M (1972) Ultrastructure of the anuran pars intermedia following severence of hypothalamic connection. Z Zellforsch 131:545–557
Dellmann HD, Owsley PA (1969) Investigations on the hypothalamo-neurohypophysial neurosecretory system of the grass-frog (Rana pipiens) after transection of the proximal neurohypophysis. II. Light- and electron-microscopic findings in the disconnected distal neurohypophysis with special emphasis on the pituicytes. Z Zellforsch 94:325–336
Dellmann HD, Stoeckel ME, Porte A, Stutinsky F, Chang N, Adldinger HK (1974) Herring bodies reexamined: an ultrastructural investigation of the rat neural lobe. Anat Histol Embryol 3:101–110
Farquhar MG (1971) Processing of secretory products by cells of the anterior pituitary gland. Mem Soc Endocrinol 19:79–122
Farquhar MG, Skutelsky EH, Hopkins CR (1975) Structure and function of the anterior pituitary and dispersed pituitary cells. In vitro studies. In: Tixier-Vidal A, Farquhar MG (eds) The Anterior Pituitary. Academic Press, New York, pp 83–135
Forbes MS (1972) Observations on the fine structure of the pars intermedia in the lizard Anolis carolinensis. Gen Comp Endocrinol 18:146–161
Franzoni MF, Fasolo A (1975) Fine structural changes induced by 6-hydroxydopamine in the pars intermedia of the newt. J Submicrosc Cytol 7:107–119
Hopkins CR (1971) Localization of adrenergic fibers in the amphibian pars intermedia by electron microscope autoradiography and their selected removal by 6-hydroxydopamine. Gen Comp Endocrinol 16:112–120
Howe A, Maxwell DS (1968) Electron microscopy of the pars intermedia of the pituitary gland in the rat. Gen Comp Endocrinol 11:169–185
Larsson L, Rodriguez EM, Meurling P (1979) Control of the pars intermedia of the lizard, Anolis carolinensis. III. Changes in the ultrastructure of the disconnected neuro-intermediate lobe. Cell Tissue Res 199:1–23
Leatherland JF (1970) Seasonal variation in the structure and ultrastructure of the pituitary of the marine form (Trachurus) of the threespine stickleback, Gasterosteus aculeatus L. I. Rostral pars distalis. Z Zellforsch 104:301–307
Lemkey-Johnston N, Bulter V, Reynolds WA (1976) Glial changes in the progress of a chemical lesion. An electron microscopic study. J Comp Neurol 167:481–502
Pearson AK, Licht P (1974) Embryology and cytodifferentiation of the pituitary gland in the lizard Anolis carolinensis. J Morphol 144:85–118
Pehlemann FW (1967) Ultrastructure and innervation of the pars intermedia of the pituitary of Xenopus laevis. Gen Comp Endocrinol 9:481
Perryman EK (1974) Fine structure of the secretory activity of the pars intermedia of Rana pipiens. Gen Comp Endocrinol 23:94–110
Perryman EK (1975) Ultrastructure of the stellate cell in the pars intermedia in the frog, Rana pipiens. Cell Tissue Res 164:387–399
Perryman EK (1976) Permeability of the amphibian pars intermedia to peroxidase injected intravascularly. Cell Tissue Res 173:401–405
Perryman EK, Bagnara JT (1978) Extravascular transfer within the anuran pars intermedia. Cell Tissue Res 193:297–313
Rawdon BB (1978) Ultrastructure of the nongranulated hypophysial cells in the teleost Pseudocrenilabrus philander (Hemihaplochromis philander), with particular reference to cytological changes in culture. Acta Zool (Stockh) 59:25–33
Saland LC (1968) Ultrastructure of the frog pars intermedia in relation to hypothalamic control of hormone release. Neuroendocrinology 3:72–88
Semoff S, Hadley ME (1978) Localization of ATPase activity in the glial-like cells of the pars intermedia. Gen Comp Endocrinol 35:329–341
Varon SS, Somjen GG (1979) Neuron-glia interactions. Neurosci Res Progr Bull 17:33–39
Weatherhead B (1971) Cytology of the neuro-intermediate lobe of the tuatara, Sphenodon punctatus, Gray. Z Zellforsch 119:21–42
Weiss M (1965) The release of pituitary secretion in the platyfish, Xiphophorus maculatus (Guenther). Z Zellforsch 68:783–794
Yamashita K (1969) Electron microscopic observations on the postnatal development of the anterior pituitary of the mouse. Gunma Symp Endocrinol 6:177–196
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Supported by NSF Program for Small College Faculty Engaged in Research at Larger Institutions and Department of Energy — Associated Western Universities Faculty Participation Program. The authors thank Dr. W. Ferris and Dr. J. Berliner for the use of the electron microscopy facilities at the University of Arizona and Nuclear Medicine Laboratory, UCLA, respectively. Warm thanks are due to Ms. Ruth Cole for technical assistance
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Perryman, E.K., de Vellis, J. & Bagnara, J.T. Phagocytic activity of the stellate cells in the anuran pars intermedia. Cell Tissue Res. 208, 85–98 (1980). https://doi.org/10.1007/BF00234175
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DOI: https://doi.org/10.1007/BF00234175