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Messenger RNA in regenerating liver: implications for the understanding of regulated growth

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Summary

The application of nucleic acid hybridization techniques to the study of liver regeneration has led to a revision of some well-established ideas about the patterns of gene expression during regenerative growth. This paper focuses on two broad problems: a) the extent to which mRNA populations in regenerating liver differ qualitatively or quantitatively from those of normal liver, and b) the similarities and differences between the pattern of gene expression during liver regeneration and liver development. Answers to these questions have come from studies in normal and regenerating liver of, a) the proportion of non-repetitive and repetitive DNA transcribed, b) the complexity of mRNA populations and the abundance of sequences in these populations, c) the extent of homology between mRNA populations, d) the amounts of specific mRNAs for albumin, alphafetoprotein, and fibrinogen, and e) the transcription of some cellular oncogenes.

Changes in the abundance of liver mRNA transcripts, without major qualitative alterations in the spectrum of sequences contained in the RNA populations, are sufficient to permit the transition of hepatocytes from a resting into a dividing state. Transcripts from at least two cellular oncogenes are included among the mRNA sequences which become more abundant during liver regeneration. Analysis of the expression of some specific genes (albumin, alphafetoprotein and fibrinogen) during liver regeneration suggests that there is little similarity between the patterns of gene expression in regenerating and developing liver.

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  1. Division of Biology and Medicine, Department of Pathology, Brown University, R102912, Providence, U.S.A.

    Nelson Fausto

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Fausto, N. Messenger RNA in regenerating liver: implications for the understanding of regulated growth. Mol Cell Biochem 59, 131–147 (1984). https://doi.org/10.1007/BF00231309

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