Summary
Opsin-like immunoreactivity was observed in the retinae and pineal organs of the mouse, rat and guinea pig, and the pineal organ of the cat. In the retina the immunoreaction was restricted to photoreceptor cells, which displayed immunostaining in their perikarya and outer and inner segments. Distinct pinealocytes endowed with characteristic processes were labelled in the pineal organs of the mouse and cat. However, in the cat the number of immunoreactive pinealocytes was very limited. In the pineal organs of the rat and guinea pig immunoreaction was very weak and diffuse. No immunoreaction was observed when the antibody was preabsorbed with purified bovine (rhod)opsin. These findings are in accord with the results of previous studies indicating molecular similarities between retinal photoreceptors and pinealocytes in mammals.
Similar content being viewed by others
References
Chader GJ, Wiggert B (1985) Interphotoreceptor retinoid-binding protein (IRBP): a link between retinal rod cells and pineal gland? In: O'Brien P, Klein DC (eds) Pineal-retinal relationships. Academic Press, New York (in press)
Collin JP (1971) Differentiation and regression of the cells of the sensory line in the epiphysis cerebri. In: Wolstenholme GEW, Knight J (eds) The pineal gland. Churchill-Livingstone, London-Edinburgh, pp 79–125
Collin JP, Oksche A (1981) Structural and functional relationships in the nonmammalian pineal organ. In: Reiter RJ (ed) The pineal gland. Vol. 1. CRC-Press, Boca Raton, pp 27–47
Fekete DM, Barnstable CJ (1983) The subcellular localization of rat photoreceptor-specific antigens. J Neurocytol 12:785–803
Grip WJ de (1982) Purification of bovine rhodopsin over concanavalin A-sepharose. Methods Enzymol 81:197–207
Grip WJ de, Daemen FJM, Bonting SL (1980) Isolation and purification of bovine rhodopsin. Methods Enzymol 67:301–320
Kalsow CM, Wacker WB (1977) Pineal reactivity of anti-retina sera. Invest Ophthalmol Visual Sci 16:181–186
Korf HW, Møller M, Gery I, Zigler JS, Klein DC (1985) Immunocytochemical demonstration of retinal S-antigen in the pineal organ of four mammalian species. Cell Tissue Res 239:81–85
Kühn H, Dreyer WJ (1972) Light dependent phosphorylation of rhodopsin by ATP. FEBS Lett 20:1–6
Margry RJCF, Jacobs CWM, Bonting SL, Grip WJ de, Daemen FJM (1983) Detergent-induced specificity of an anti-rhodopsin serum for opsin micro-complement fixation studies. Biochim Biophys Acta 742:463–470
Mirshahi M, Faure JP, Brisson P, Falcon J, Guerlotte J, Collin JP (1984) S-antigen immunoreactivity in retinal rods and cones and pineal photosensitive cells. Biol Cell 52:195–198
Møller M, Glistrup OV, Olsen W (1983) Contrast enhancement of the brownish horseradish peroxidase activated 3,3′-diaminobenzidine tetrahydrochloride reaction product in black and white photomicrography by the use of interference filters. J Histochem Cytochem 32:37–42
Oksche A (1971) Sensory and glandular elements of the pineal organ. In: Wolstenholme GEW, Knight J (eds) The pineal gland. Churchill-Livingstone, Edinburgh London, pp 127–146
Schalken JJ, Janssen JJM, Grip WJ de, Hawkins RK, Sanyal S (1985) Immunoassay of rod visual pigment (opsin) in the eyes of rds mutant mice lacking receptor outer segments. Biochim Biophys Acta 839:122–126
Somers RL, Klein DC (1985) Rhodopsin kinase activity in the mammalian pineal gland and other tissues. Science 226:182
Sternberger LA (1979) Immunocytochemistry. John Wiley Sons, New York Chichester Brisbane Toronto
Vigh B, Vigh-Teichmann I (1981) Light- and electron-microscopic demonstration of immunoreactive opsin in the pinealocytes of various vertebrates. Cell Tissue Res 221: 451–463
Author information
Authors and Affiliations
Additional information
Supported by grants from the Deutsche Forschungsgemeinschaft to HWK, the European Science Foundation to RGF, the Alexander-von-Humboldt Stiftung to PE, and the Dutch Foundation for the Advancement of Basic Research. The authors are greatly indebted to Dr. Willem de Grip, Nijmegen, and Profesor Andreas Oksche, Giessen, for their critical interest in this study
Rights and permissions
About this article
Cite this article
Korf, H.W., Foster, R.G., Ekström, P. et al. Opsin-like immunoreaction in the retinae and pineal organs of four mammalian species. Cell Tissue Res. 242, 645–648 (1985). https://doi.org/10.1007/BF00225432
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225432